朝向无 Matrigel 的类器官培养。
Towards organoid culture without Matrigel.
机构信息
DEVCOM US Army Research Laboratory, Weapons and Materials Research Directorate, Science of Extreme Materials Division, Polymers Branch, 6300 Rodman Rd. Building 4600, Aberdeen Proving Ground, Aberdeen, MD, 21005, USA.
Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope National Medical Center, 1500 Duarte Rd., Duarte, CA, 91010, USA.
出版信息
Commun Biol. 2021 Dec 10;4(1):1387. doi: 10.1038/s42003-021-02910-8.
Organoids-cellular aggregates derived from stem or progenitor cells that recapitulate organ function in miniature-are of growing interest in developmental biology and medicine. Organoids have been developed for organs and tissues such as the liver, gut, brain, and pancreas; they are used as organ surrogates to study a wide range of questions in basic and developmental biology, genetic disorders, and therapies. However, many organoids reported to date have been cultured in Matrigel, which is prepared from the secretion of Engelbreth-Holm-Swarm mouse sarcoma cells; Matrigel is complex and poorly defined. This complexity makes it difficult to elucidate Matrigel-specific factors governing organoid development. In this review, we discuss promising Matrigel-free methods for the generation and maintenance of organoids that use decellularized extracellular matrix (ECM), synthetic hydrogels, or gel-forming recombinant proteins.
类器官是由干细胞或祖细胞衍生而来的细胞聚集体，可在微型化中再现器官功能，在发育生物学和医学领域引起了越来越多的关注。已经开发出用于肝脏、肠道、大脑和胰腺等器官和组织的类器官；它们被用作器官替代物，用于研究基础和发育生物学、遗传疾病和治疗等广泛的问题。然而，迄今为止报道的许多类器官都是在 Matrigel 中培养的，Matrigel 是从小鼠肉瘤细胞 Engelbreth-Holm-Swarm 的分泌产物中制备的；Matrigel 复杂且定义不明确。这种复杂性使得阐明控制类器官发育的 Matrigel 特异性因素变得困难。在这篇综述中，我们讨论了有前途的无 Matrigel 方法，用于生成和维持使用脱细胞细胞外基质 (ECM)、合成水凝胶或凝胶形成重组蛋白的类器官。
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