Stastny J J, Fosslien E
Department of Pathology, University of Illinois, College of Medicine, Chicago 60612.
Exp Mol Pathol. 1992 Dec;57(3):205-14. doi: 10.1016/0014-4800(92)90011-y.
Proteins from grossly and histologically normal human aortic intimas and human aortic intima with fatty streaks or fibro-fatty lesions were extracted with 9 M urea mixture. Protein extracts were mixed with an internal absorbance calibrator (carbonic anhydrase) and subsequently separated by two-dimensional gel electrophoresis, silver stained, and quantitated by a laser beam densitometer. The vascular-origin proteins actin, tropomyosin-like proteins, tubulin, glycoprotein G35, and two myosin light chains were present in the highest amounts in normal aortic intima (27-year-old male). Quantitation of vascular-origin proteins in aortic intima with a fibro-fatty lesion from the same subject showed a slight decrease in relative amount of these proteins as compared to the normal intima. Several polypeptides (P15, P18, P60, P110b) and plasma-derived proteins not observed in the normal intima were found in fibro-fatty lesion (albumin, haptoglobin beta-chain, fibrinogen beta-chain, alpha 1-HS-glycoprotein). Other proteins which were present in very low amounts in the normal intima (transferrin, alpha 1-antitrypsin, apolipoprotein A-1, P56, P190) were found to be major proteins of intima with fibro-fatty lesion. Differences in relative amount of plasma-derived and vascular-origin proteins between normal intima and intima with fatty streaks, studied in a large number of specimens from 38 thoracic intimas and 18 paired abdominal intimas (16-34 years old) were less prominent. Statistically significant increases of the albumin/actin ratio were found in fatty streaks as compared to paired normal intimas as well as in the mean value of albumin/actin ratio in the group of fibro-fatty lesions (mean = 6.1) as compared to the group of fatty streaks (mean = 1.7) or normal intima (mean = 0.7). Several lesion unique proteins were observed; however, the frequency of the occurrence of these proteins in 41 specimens with lesion was low. No significant differences were observed in intima protein pattern and quantities of selected intima proteins between paired thoracic and abdominal aortas.
用9M尿素混合物提取大体及组织学正常的人主动脉内膜、伴有脂肪条纹或纤维脂肪病变的人主动脉内膜中的蛋白质。蛋白质提取物与内部吸光度校准物（碳酸酐酶）混合，随后通过二维凝胶电泳进行分离、银染，并通过激光束密度计进行定量。血管源性蛋白肌动蛋白、原肌球蛋白样蛋白、微管蛋白、糖蛋白G35和两条肌球蛋白轻链在正常主动脉内膜（27岁男性）中的含量最高。对同一受试者伴有纤维脂肪病变的主动脉内膜中的血管源性蛋白进行定量分析，结果显示与正常内膜相比，这些蛋白的相对含量略有下降。在纤维脂肪病变中发现了几种正常内膜中未观察到的多肽（P15、P18、P60、P110b）和血浆衍生蛋白（白蛋白、触珠蛋白β链、纤维蛋白原β链、α1-HS-糖蛋白）。在正常内膜中含量极低的其他蛋白（转铁蛋白、α1-抗胰蛋白酶、载脂蛋白A-1、P56、P190）被发现是伴有纤维脂肪病变的内膜的主要蛋白。在大量来自38例胸主动脉内膜和18对腹主动脉内膜（16 - 34岁）的标本中研究发现，正常内膜与伴有脂肪条纹的内膜之间血浆衍生蛋白和血管源性蛋白相对含量的差异不太显著。与配对正常内膜相比，脂肪条纹中白蛋白/肌动蛋白比值有统计学意义的升高，与脂肪条纹组（平均值 = 1.7）或正常内膜组（平均值 = 0.7）相比，纤维脂肪病变组中白蛋白/肌动蛋白比值的平均值（平均值 = 6.1）也有统计学意义的升高。观察到几种病变特异性蛋白；然而，这些蛋白在41例有病变的标本中的出现频率较低。配对的胸主动脉和腹主动脉内膜在蛋白质模式和选定内膜蛋白的数量上未观察到显著差异。
