Stastny J, Robertson A L, Fosslien E
Exp Mol Pathol. 1986 Dec;45(3):279-86. doi: 10.1016/0014-4800(86)90016-x.
The protein composition of atheroma-free human thoracic intima was compared with that containing fatty streaks or fibro-fatty lesions utilizing two-dimensional gel electrophoresis (2-DE) and silver staining. Intimal proteins extracted with 9 M urea were separated by nonequilibrium pH gradient electrophoresis (NEPHGE) followed by polyacrylamide gel electrophoresis (PAGE) in the second dimension. NEPHGE-PAGE of proteins extracted from atheroma-free intima revealed several major proteins: actin, tropomyosin-like proteins, proteins with relative molecular weight (Mr) of 250,000 (P250), two proteins with Mr about 15,000 (P15a, P15b), and many medium proteins such as a myosin heavy chain, two myosin light chains, and proteins P47, P44, P32, P27, P20a, P20b, P19a, P19b. Several additional proteins were observed in intimas with fatty streaks and fibro-fatty lesions. Most of them, such as albumin, transferrin, Apo A-I, alpha 1-antitrypsin, fibrinogen beta-chain, IgG, appear to originate from plasma. Differences in protein composition of intima with fibro-fatty streaks compared with adjacent lesion-free intima varied from case to case and need further study. NEPHGE-PAGE in combination with isoelectric focusing (ISO)-PAGE revealed more intimal proteins in atheroma-free and diseased aortas than either method alone, proteins which might be quantitated, isolated for binding studies, and further evaluated for their potential role in atherogenesis.
利用二维凝胶电泳（2-DE）和银染法，对无动脉粥样硬化的人胸段内膜与含有脂肪条纹或纤维脂肪病变的内膜的蛋白质组成进行了比较。用9M尿素提取的内膜蛋白先通过非平衡pH梯度电泳（NEPHGE）分离，然后在第二维进行聚丙烯酰胺凝胶电泳（PAGE）。从无动脉粥样硬化内膜提取的蛋白质进行NEPHGE-PAGE分析，显示出几种主要蛋白质：肌动蛋白、原肌球蛋白样蛋白质、相对分子质量（Mr）为250,000的蛋白质（P250）、两种Mr约为15,000的蛋白质（P15a、P15b），以及许多中等大小的蛋白质，如肌球蛋白重链、两种肌球蛋白轻链，还有蛋白质P47、P44、P32、P27、P20a、P20b、P19a、P19b。在有脂肪条纹和纤维脂肪病变的内膜中还观察到几种其他蛋白质。其中大多数，如白蛋白、转铁蛋白、载脂蛋白A-I、α1-抗胰蛋白酶、纤维蛋白原β链、IgG，似乎源自血浆。与相邻无病变内膜相比，有纤维脂肪条纹的内膜蛋白质组成差异因病例而异，需要进一步研究。NEPHGE-PAGE与等电聚焦（ISO）-PAGE相结合，在无动脉粥样硬化和患病的主动脉中发现的内膜蛋白质比单独使用任何一种方法都多，这些蛋白质可以进行定量、分离用于结合研究，并进一步评估它们在动脉粥样硬化发生中的潜在作用。
