Florêncio M S, Peixoto C A
Departamento de Patologia e Biologia Celular, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), and Laboratório de Imunopatologia Keizo Asami da Universidade Federal de Pernambuco, Recife, Brazil.
Parasitology. 2003 Jun;126(Pt 6):551-4.
An ultrastructural study of microfilariae of Wuchereria bancrofti was performed after in vitro treatment with diethylcarbamazine. One of the first morphological alterations produced by treatment with 5, 10 or 50 microg/ml of DEC was the loss of microfilarial sheaths. Drastic effects of DEC were only detected when microfilariae were exsheathed. Microfilariae treated with 5, 10 or 50 microg/ml DEC showed severely affected organelles, formation of several vacuoles mainly in the hypodermis, and cytolysis. Some microfilariae showed extreme cellular disorganization with abundance of electron-dense degenerating organelles, numerous large vacuoles, and nuclear condensation. Lamellar bodies probably related to an assembly of endoplasmic reticulum membranes were observed in some damaged microfilariae. Thus, in vitro treatment with concentrations of DEC similar to therapeutic conditions had direct effect by causing a loss of the microfilarial sheath of W. bancrofti with subsequent damage of organelles and apoptosis.
在用乙胺嗪进行体外处理后，对班氏吴策线虫微丝蚴进行了超微结构研究。用5、10或50微克/毫升的乙胺嗪处理后产生的首批形态学改变之一是微丝蚴鞘的丧失。仅当微丝蚴脱鞘时才检测到乙胺嗪的显著作用。用5、10或50微克/毫升乙胺嗪处理的微丝蚴显示细胞器受到严重影响，主要在皮下形成多个液泡，并出现细胞溶解。一些微丝蚴表现出极端的细胞紊乱，有大量电子致密的退化细胞器、众多大液泡和核浓缩。在一些受损的微丝蚴中观察到可能与内质网膜组装有关的板层小体。因此，在体外以与治疗条件相似的浓度用乙胺嗪处理会产生直接作用，导致班氏吴策线虫微丝蚴鞘丧失，随后细胞器受损并发生凋亡。
