So L M, Chu L M, Wong P K
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China.
Chemosphere. 2003 Sep;52(9):1499-503. doi: 10.1016/S0045-6535(03)00488-0.
Bacteria resistant to Cu2+, Ni2+ or Zn2+ were isolated from the rhizosphere of water hyacinth (Eichhornia crassipes (Mart.)) and their metal ion removal capacities (RCs) were determined. The Ni2+ and Zn2+ RCs of the respective metal ion-resistant bacteria were less than 4.1 mg g(-1), while one of the Cu2+-resistant bacteria (Strain CU-1) showed a significant high Cu2+ RC of 10.6 mg g(-1). The effect of inoculating water hyacinth with Strain CU-1 on its Cu2+ RC was further studied. Water hyacinths were treated with an antibiotic, oxytetracycline (OTC), to remove most rhizospheric bacteria of plant roots. Inoculation of Strain CU-1 increased the Cu2+ RC of the plant root by 1.91 (OTC-treated) and 1.56 (OTC-untreated) folds respectively when compared with the control. Results also showed that Strain CU-1 colonized onto the plant root and led to the increase of Cu2+ RC of the roots of water hyacinth.
从凤眼莲（Eichhornia crassipes (Mart.)）根际分离出对Cu2+、Ni2+或Zn2+具有抗性的细菌，并测定了它们的金属离子去除能力（RCs）。各自的金属离子抗性细菌对Ni2+和Zn2+的RCs小于4.1 mg g(-1)，而其中一种抗Cu2+细菌（菌株CU-1）表现出显著高的Cu2+ RC，为10.6 mg g(-1)。进一步研究了用菌株CU-1接种凤眼莲对其Cu2+ RC的影响。用水生抗生素土霉素（OTC）处理凤眼莲，以去除植物根部的大多数根际细菌。与对照相比，接种菌株CU-1使植物根的Cu2+ RC分别增加了1.91倍（经OTC处理）和1.56倍（未经OTC处理）。结果还表明，菌株CU-1定殖在植物根部，导致凤眼莲根部的Cu2+ RC增加。
