Huguenot D, Bois P, Cornu J Y, Jezequel K, Lollier M, Lebeau T
Equipe Dépollution Biologique des Sols, Université de Haute Alsace, EA 3991 LVBE (Laboratoire Vigne Biotechnologies et Environnement), 33 rue de Herrlisheim, BP 50568 68008, Colmar, France,
Environ Sci Pollut Res Int. 2015 Jan;22(1):721-32. doi: 10.1007/s11356-014-3406-6. Epub 2014 Aug 10.
The use of plants and microorganisms to mitigate sediment contaminated by copper was studied in microcosms that mimic the functioning of a stormwater basin (SWB) connected to vineyard watershed. The impact of phytoremediation and bioaugmentation with siderophore-producing bacteria on the fate of Cu was studied in two contrasted (batch vs. semi-continuous) hydraulic regimes. The fate of copper was characterised following its discharge at the outlet of the microcosms, its pore water concentration in the sediment, the assessment of its bioaccessible fraction in the rhizosphere and the measurement of its content in plant tissues. Physico-chemical (pH, redox potential) and biological parameters (total heterotrophic bacteria) were also monitored. As expected, the results showed a clear impact of the hydraulic regime on the redox potential and thus on the pore water concentration of Cu. Copper in pore water was also dependent on the frequency of Cu-polluted water discharges. Repeated bioaugmentation increased the total heterotrophic microflora as well as the Cu bioaccessibility in the rhizosphere and increased the amount of Cu extracted by Phragmites australis by a factor of ~2. Sugar beet pulp, used as a filter to avoid copper flushing, retained 20% of outcoming Cu and led to an overall retention of Cu higher than 94% when arranged at the outlet of microcosms. Bioaugmentation clearly improved the phytoextraction rate of Cu in a small-scaled SWB designed to mimic the functioning of a full-size SWB connected to vineyard watershed. Highlights: Cu phytoextraction in constructed wetlands much depends on the hydraulic regime and on the frequency of Cu-polluted water discharges. Cu phytoextraction increases with time and plant density. Cu bioaccessibility can be increased by bioaugmentation with siderophore-producing bacteria.
在模拟与葡萄园流域相连的雨水盆地（SWB）功能的微观世界中，研究了利用植物和微生物减轻铜污染沉积物的情况。在两种不同的（间歇式与半连续式）水力条件下，研究了植物修复和添加产铁载体细菌进行生物强化对铜归宿的影响。通过微观世界出口处的排放情况、沉积物孔隙水中的浓度、根际中生物可利用部分的评估以及植物组织中含量的测量来表征铜的归宿。还监测了物理化学参数（pH值、氧化还原电位）和生物学参数（总异养细菌）。正如预期的那样，结果表明水力条件对氧化还原电位有明显影响，进而对铜的孔隙水浓度产生影响。孔隙水中的铜还取决于含铜污染水的排放频率。重复进行生物强化增加了总异养微生物群落以及根际中铜的生物可利用性，并使芦苇提取的铜量增加了约2倍。用作过滤器以避免铜被冲走的甜菜浆保留了20%的流出铜，当放置在微观世界出口处时，导致铜的总体保留率高于94%。在一个旨在模拟与葡萄园流域相连的全尺寸SWB功能的小型SWB中，生物强化明显提高了铜的植物提取率。要点：人工湿地中铜的植物提取很大程度上取决于水力条件和含铜污染水的排放频率。铜的植物提取随时间和植物密度增加。通过添加产铁载体细菌进行生物强化可提高铜的生物可利用性。
