Mumford J A, Wood J
Animal Health Trust, Newmarket, UK.
Dev Biol Stand. 1992;79:137-46.
Shortcomings in the original methods (based on haemagglutination of erythrocytes) used to measure potency of equine influenza vaccines and antibody responses stimulated by vaccines, coupled with the lack of a reliable challenge system in the target species, has hindered progress in identifying the antigenic content required to provide protection. Reliable methods are now available for measuring the haemagglutinin (HA) content of vaccines and the antibody responses they elicit. The development of challenge systems in the target species has allowed antibody levels consistent with protection to be identified under experimental conditions. Comparison of vaccine performance under experimental conditions and in the field suggests that exposure to a nebulised virus aerosol provides a realistic challenge for the assessment of equine influenza vaccines.
用于测量马流感疫苗效力以及疫苗激发的抗体反应的原始方法（基于红细胞血凝反应）存在缺陷，再加上缺乏针对目标物种的可靠攻毒系统，阻碍了在确定提供保护所需的抗原成分方面取得进展。现在已有可靠的方法可用于测量疫苗的血凝素（HA）含量及其引发的抗体反应。目标物种攻毒系统的开发使得在实验条件下能够确定与保护作用一致的抗体水平。实验条件下和实际应用中疫苗性能的比较表明，暴露于雾化病毒气溶胶可为评估马流感疫苗提供现实的攻毒方式。
