Darwich Laila, Pié Sandrine, Rovira Albert, Segalés Joaquim, Domingo Mariano, Oswald Isabelle P, Mateu Enric
Centre de Recerca en Sanitat Animal (CReSA), Departament de Sanitat i d'Anatomia Animals (Unitat de Malalties Infeccioses), Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Institut National de Recherche Agronomique, Laboratoire de Pharmacologie et Toxicologie, 180 chemin de Tournefeuille, BP3, 31931 Toulouse, France.
J Gen Virol. 2003 Aug;84(Pt 8):2117-2125. doi: 10.1099/vir.0.19124-0.
Fifteen 8-week-old conventional pigs were selected from a farm where pigs were suffering from postweaning multisystemic wasting syndrome (PMWS). Ten of the animals were diseased pigs showing typical signs of PMWS (wasting and respiratory disorders) and positive for infection with porcine circovirus type 2 (PCV2), and the other five animals selected as controls were pen-mate, apparently healthy pigs. Blood samples and lymphoid tissues were taken from each animal for haematological, serological and histopathological studies. Also, cytokine mRNA expression of IL-1beta, IL-2, IL-4, IL-8, IL-10, IL-12p40 and IFN-gamma from inguinal and bronchial lymph nodes, tonsils, spleen and thymus was determined by semi-quantitative RT-PCR. Pigs suffering from PMWS showed severe alterations of haematological parameters such as anaemia, lymphopenia with decrease of CD8(+) and IgM(+) cells, monocytosis and neutrophilia. Also, extensive lymphocyte depletion and altered cytokine mRNA expression patterns were seen in most of the examined lymphoid organs. Those cytokine mRNA alterations were characterized by an overexpression of IL-10 mRNA in thymus and IFN-gamma mRNA in tonsils, and by decreases in the mRNA expression of several cytokines as IL-2 and IL-12p40 in the spleen, IL-4 in tonsils, and IFN-gamma, IL-10, IL-12p40 and IL-4 in inguinal lymph nodes. Also, the IL-10 mRNA overexpression was histologically associated with the thymic depletion and atrophy observed in PMWS pigs. In conclusion, the cytokine mRNA imbalance, specially the increased mRNA levels of IL-10 in the thymus, jointly with the histopathological and haematological disorders, are highly indicative of a T-cell immunosuppression, enhancing the notion that the immune system of PMWS-affected pigs is severely impaired.
从一个猪群中挑选出15头8周龄的普通猪，该猪群正遭受断奶后多系统消耗综合征（PMWS）。其中10头为患病猪，表现出PMWS的典型症状（消瘦和呼吸紊乱），且猪圆环病毒2型（PCV2）感染呈阳性，另外5头被选作对照的猪是同栏饲养、表面健康的猪。从每头猪采集血液样本和淋巴组织用于血液学、血清学和组织病理学研究。此外，通过半定量逆转录聚合酶链反应（RT-PCR）测定腹股沟和支气管淋巴结、扁桃体、脾脏和胸腺中白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、白细胞介素-12p40和干扰素-γ（IFN-γ）的细胞因子mRNA表达。患有PMWS的猪表现出血液学参数的严重改变，如贫血、淋巴细胞减少伴CD8(+)和IgM(+)细胞减少、单核细胞增多和中性粒细胞增多。此外，在大多数检查的淋巴器官中可见广泛的淋巴细胞耗竭和细胞因子mRNA表达模式改变。这些细胞因子mRNA改变的特征是胸腺中IL-10 mRNA过表达、扁桃体中IFN-γ mRNA过表达，以及脾脏中IL-2和IL-12p40、扁桃体中IL-4、腹股沟淋巴结中IFN-γ、IL-10、IL-12p40和IL-4的mRNA表达降低。此外，IL-10 mRNA过表达在组织学上与PMWS猪中观察到的胸腺耗竭和萎缩相关。总之，细胞因子mRNA失衡，特别是胸腺中IL-10 mRNA水平升高，与组织病理学和血液学紊乱共同高度提示T细胞免疫抑制，强化了受PMWS影响的猪免疫系统严重受损的观点。
