Shingai Masashi, Ayata Minoru, Ishida Hiroshi, Matsunaga Isamu, Katayama Yuko, Seya Tsukasa, Tatsuo Hironobu, Yanagi Yusuke, Ogura Hisashi
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Department of Pediatrics, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
J Gen Virol. 2003 Aug;84(Pt 8):2133-2143. doi: 10.1099/vir.0.19091-0.
The vaccine or Vero cell-adapted strains of measles virus (MV) have been reported to use CD46 as a cell entry receptor, while lymphotropic MVs preferentially use the signalling lymphocyte activation molecule (SLAM or CD150). In contrast to the virus obtained from patients with acute measles, little is known about the receptor that is used by defective variants of MV isolated from patients with subacute sclerosing panencephalitis (SSPE). The receptor-binding properties of SSPE strains of MV were analysed using vesicular stomatitis virus pseudotypes expressing the envelope glycoproteins of SSPE strains of MV. Such pseudotype viruses could use SLAM but not CD46 for entry. The pseudotype viruses with SSPE envelope glycoproteins could enter Vero cells, which do not express SLAM. In addition, their entry was not blocked by the monoclonal antibody to CD46, pointing to another entry receptor for SSPE strains on Vero cells. Furthermore, the unknown receptor(s), distinct from SLAM and CD46, may be present on cell lines derived from lymphoid and neural cells. Biochemical characterization of the receptor present on Vero cells and SK-N-SH neuroblastoma cells was consistent with a glycoprotein. Identification of additional entry receptors for MV will provide new insights into the mechanism of spread of MV in the central nervous system and possible reasons for differences between MVs isolated from patients with acute measles and SSPE.
据报道，麻疹病毒（MV）的疫苗株或Vero细胞适应株利用CD46作为细胞进入受体，而嗜淋巴细胞性MV则优先利用信号淋巴细胞激活分子（SLAM或CD150）。与从急性麻疹患者分离得到的病毒不同，对于从亚急性硬化性全脑炎（SSPE）患者分离得到的MV缺陷变异株所使用的受体了解甚少。使用表达MV的SSPE株包膜糖蛋白的水泡性口炎病毒假型分析了MV的SSPE株的受体结合特性。这种假型病毒可利用SLAM而非CD46进入细胞。带有SSPE包膜糖蛋白的假型病毒可进入不表达SLAM的Vero细胞。此外，它们的进入不受抗CD46单克隆抗体的阻断，这表明Vero细胞上存在SSPE株的另一种进入受体。此外，不同于SLAM和CD46的未知受体可能存在于源自淋巴和神经细胞的细胞系上。对Vero细胞和SK-N-SH神经母细胞瘤细胞上存在的受体进行生化特性分析，结果与一种糖蛋白一致。鉴定MV的其他进入受体将为MV在中枢神经系统中的传播机制以及从急性麻疹患者和SSPE患者分离得到的MV之间差异的可能原因提供新的见解。
