通过多重半巢式聚合酶链反应同时检测和区分公猪精液中的猪圆环病毒和猪细小病毒。

Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction.

作者信息

Kim Junghyun, Han Dong Un, Choi Changsun, Chae Chanhee

机构信息

Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Republic of Korea.

出版信息

J Vet Med Sci. 2003 Jun;65(6):741-4. doi: 10.1292/jvms.65.741.

DOI:10.1292/jvms.65.741

PMID:12867738

Abstract

A multiplex seminested polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among porcine circovirus 1 (PCV1), PCV2, and porcine parvovirus (PPV) from boar semen. Primers of PCV1, PCV2 and PPV were specific and did not react with other viruses respectively. Twenty (20.4%) and 42 (42.9%) out of 98 whole semen samples were found to be positive for PCV and PPV using multiplex conventional and seminested PCR, respectively. When the separated fractions of PCV or PPV-contaminated semen were analyzed using multiplex seminested PCR, PCV and PPV DNA were found to be present mainly in the seminal fluid and nonsperm cell fractions. This multiplex seminested PCR assay was sensitive, rapid and a good alternative method for the detection and differentiation of these viruses in boar semen.

摘要

开发了一种多重半巢式聚合酶链反应（PCR），用于同时检测和区分公猪精液中的猪圆环病毒1型（PCV1）、猪圆环病毒2型（PCV2）和猪细小病毒（PPV）。PCV1、PCV2和PPV的引物具有特异性，分别不与其他病毒发生反应。使用多重常规PCR和半巢式PCR分别检测98份全精液样本，发现其中20份（20.4%）PCV呈阳性，42份（42.9%）PPV呈阳性。当使用多重半巢式PCR分析PCV或PPV污染精液的分离组分时，发现PCV和PPV DNA主要存在于精液和非精子细胞组分中。这种多重半巢式PCR检测方法灵敏、快速，是检测和区分公猪精液中这些病毒的良好替代方法。