Chapman Elisabeth J, Hilson Pierre, German Thomas L
Department of Plant Pathology, University of Wisconsin-Madison, 53706, USA.
Intervirology. 2003;46(3):177-81. doi: 10.1159/000071459.
We have previously described an in vitro assay for RNA-dependent RNA polymerase activity in virions of tomato spotted wilt virus (TSWV). Here we report antibody inhibition of virion-associated RNA synthesis in vitro with an L-protein-specific polyclonal antibody raised against the carboxy-terminus of the L protein. In contrast, RNA synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the L protein. Our results directly associate the TSWV L protein, the putative viral polymerase, with RNA synthesis functions in vitro.
我们之前描述了一种用于检测番茄斑萎病毒（TSWV）病毒粒子中RNA依赖性RNA聚合酶活性的体外测定法。在此，我们报告了用针对L蛋白羧基末端产生的L蛋白特异性多克隆抗体对体外病毒粒子相关RNA合成的抗体抑制作用。相比之下，RNA合成不受异源抗血清的抑制，且不受针对L蛋白内部部分产生的抗血清的影响。我们的结果直接将TSWV L蛋白（推测的病毒聚合酶）与体外RNA合成功能联系起来。
