de Oliveira Athos Silva, Bertran André Gustavo Machado, Inoue-Nagata Alice Kazuko, Nagata Tatsuya, Kitajima Elliot Watanabe, Oliveira Resende Renato
Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil.
Virus Genes. 2011 Dec;43(3):385-9. doi: 10.1007/s11262-011-0639-z. Epub 2011 Jul 1.
The tospoviral RNA-dependent RNA polymerases (RdRp), or L proteins, perform several conserved functions during virus replication in host cells. In this study, an L segment sequence of 9,040 bp from a new tospovirus (family Bunyaviridae) naturally infecting bean (Phaseolus vulgaris L.) plants was characterized. It encodes the largest RdRp gene known yet for this genus, with deduced 2932aa and a molecular mass of approximately 336 kDa. A Lysine-rich C-terminal extension was found, which apart from our isolate, was only recognized in another recently discovered tospovirus infecting Fabaceae, Soybean vein necrosis associated virus (SVNaV). Due to its distinct biological features and L protein-based phylogenetic analysis showing an almost equidistant position in comparison to Eurasian and American Tospovirus groups, as well as the clustering with SVNaV, we suggest the tentative name Bean necrotic mosaic virus for this unique isolate.
番茄斑萎病毒属的RNA依赖的RNA聚合酶（RdRp），即L蛋白，在病毒于宿主细胞内复制期间执行多种保守功能。在本研究中，对来自一种自然感染菜豆（Phaseolus vulgaris L.）植株的新型番茄斑萎病毒（布尼亚病毒科）的9040 bp的L片段序列进行了特征分析。它编码该属目前已知最大的RdRp基因，推导的氨基酸序列为2932个氨基酸，分子量约为336 kDa。发现了一个富含赖氨酸的C末端延伸，除了我们的分离株外，仅在另一种最近发现的感染豆科植物的番茄斑萎病毒，即大豆脉坏死相关病毒（SVNaV）中被识别。由于其独特的生物学特性以及基于L蛋白的系统发育分析显示，与欧亚和美洲番茄斑萎病毒组相比，它处于几乎等距的位置，并且与SVNaV聚类，我们建议将这种独特的分离株暂定名为菜豆坏死花叶病毒。
