Albarellos G A, Bonafine R R, Kreil V E, Ambros L A, Montoya L, Hallu R E
Area de Farmacología, Departamento de Fisiopatología y Etiopatogenia, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Chorroarín 280 (1427), Buenos Aires, Argentina.
Lab Anim. 2003 Jul;37(3):188-92. doi: 10.1258/002367703766453029.
In order to perform pharmacokinetic studies involving multiple blood sampling, repeated at variable intervals of time, a simple and reliable non-surgical jugular catheterization technique was developed. Six cats were catheterized 48 times using an indwelling through-the-needle type catheter (22G and 20.3 cm) placed into the jugular vein through an over-the-needle type (20G and 32 mm). Catheters remained in place for 1-13 days (median 3 days) without loss of patency until removal. Each jugular was catheterized a range of 2-6 times, with a total indwelling time of 4-33 days. No clinical signs of phlebitis, thrombosis or sepsis were observed either during or after the studies. This technique allows an easy, non-painful, non-stressful blood withdrawal during extended sampling periods, with minimal damage of the veins.
为了进行涉及多次采血的药代动力学研究，采血时间间隔可变，我们开发了一种简单可靠的非手术颈静脉插管技术。使用留置的穿刺针型导管（22G，长20.3厘米）通过穿刺针型（20G，长32毫米）插入颈静脉，对6只猫进行了48次插管。导管留置1 - 13天（中位数为3天），直至拔除前均保持通畅。每只颈静脉插管2 - 6次，总留置时间为4 - 33天。在研究期间及之后均未观察到静脉炎、血栓形成或败血症的临床症状。该技术可在延长的采样期内轻松、无痛、无应激地采血，对静脉的损伤最小。
