el-On J, Sneier R, Elias E
Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Isr J Med Sci. 1992 Dec;28(12):847-51.
No bacterial contamination has been demonstrated in cutaneous leishmaniasis (CL) nodule and in lesions caused by Leishmania major in Balb/c mice up to 20 days after infection. However, although many phagocytic cells (polymorphonuclear leukocytes and macrophages) were present in the CL lesion, 80% of the lesions showed bacterial contamination that developed within the first 70 days of infection. Topical treatment of the lesion with an ointment containing 15% paromomycin and 12% methylbenzethonium chloride in soft white paraffin for 20 days eliminated all the Leishmania parasites and several of the associated bacteria including: Proteus vulgaris, Pasteurella multocida, Staphylococcus albus and Staphylococcus aureus. This treatment did not affect Escherichia coli, Klebsiella spp. and Pseudomonas aeruginosa. Total elimination of these bacteria was achieved only during the healing process, and within 20 days following termination of treatment. The rate of disappearance of bacteria inoculated alone into the base of the tail of normal uninfected Balb/c mice was much faster than that of bacteria inoculated into either the CL nodule or the CL lesion. This study suggests the development of local immunosuppression in the CL lesion that may be mediated by the Leishmania parasites and their metabolites.
在皮肤利什曼病（CL）结节以及感染利什曼原虫主要种的Balb/c小鼠所产生的病变中，直至感染后20天均未发现细菌污染。然而，尽管CL病变中有许多吞噬细胞（多形核白细胞和巨噬细胞），但80%的病变在感染后的前70天内出现了细菌污染。用含有15%巴龙霉素和12%苄索氯铵的软膏在软白凡士林基质中对病变进行局部治疗20天，消除了所有利什曼原虫寄生虫以及几种相关细菌，包括：普通变形杆菌、多杀巴斯德菌、白色葡萄球菌和金黄色葡萄球菌。这种治疗方法对大肠杆菌、克雷伯菌属和铜绿假单胞菌没有影响。只有在愈合过程中以及治疗结束后的20天内，这些细菌才被完全清除。单独接种到正常未感染的Balb/c小鼠尾巴根部的细菌消失速度比接种到CL结节或CL病变中的细菌快得多。这项研究表明，CL病变中可能由利什曼原虫寄生虫及其代谢产物介导产生了局部免疫抑制。
