el-On J, Sulitzeanu A, Schnur L F
Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheba, Israel.
Ann Trop Med Parasitol. 1991 Jun;85(3):323-8. doi: 10.1080/00034983.1991.11812568.
Cutaneous lesions caused by Leishmania major in BALB/c mice were cured completely when treated topically with an ointment comprising 15% paromomycin sulphate and 1-2% methylbenzethonium chloride ointment in soft white paraffin twice daily for 10 days. No parasites were detected in tissue smears or in cultures from treated cutaneous lesions. Re-developing lesions, considered to be resulting from the migration of parasites from internal organs, showed almost the same response to topical treatment. Promastigotes of the virulent clone 121 of L. major LRC-L137 which were exposed to 100 micrograms ml-1 of paromomycin in RPMI medium at 28 degrees C developed resistance to the drug over 10 passages of exposure. Enzyme analysis of susceptible and resistant promastigotes of this clone showed no differences with regard to their profiles based on 11 enzymes.
当用一种软膏局部治疗时，感染硕大利什曼原虫的BALB/c小鼠的皮肤损伤完全治愈。该软膏由15%硫酸巴龙霉素和1-2%甲基苄索氯铵溶于软白凡士林组成，每天涂抹两次，持续10天。在处理过的皮肤损伤的组织涂片或培养物中未检测到寄生虫。重新出现的损伤被认为是由寄生虫从内部器官迁移引起的，对局部治疗表现出几乎相同的反应。在28℃下，将硕大利什曼原虫LRC-L137的强毒株克隆121的前鞭毛体暴露于含100微克/毫升巴龙霉素的RPMI培养基中，经过10代传代后，对该药物产生了抗性。对该克隆的敏感和抗性前鞭毛体进行酶分析，基于11种酶的图谱显示没有差异。
