通过调控序列重复工程改造的蛋白质中的长距离构象变化
Long-distance conformational changes in a protein engineered by modulated sequence duplication.
作者信息
Sagermann Martin, Gay Leslie, Matthews Brian W
机构信息
Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, OR 97403-1229, USA.
出版信息
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. doi: 10.1073/pnas.1633549100. Epub 2003 Jul 17.
Abstract
There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.
摘要
几乎没有已知的实例表明生物信号是通过蛋白质主体的大的构象变化进行传递的。我们在此描述一种经过工程改造的T4溶菌酶突变体，其能够在一定距离上实现结构变化。该设计使用了串联序列重复，使得在17 - 25埃的距离上，能够将大规模的结构变化从α螺旋的一端传递到另一端。该方法应具有普遍适用性，并且可能使得在蛋白质的一个位点引入突变，从而在空间上较远的位点诱导结构发生大规模变化成为可能。
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