通过流式细胞术分析RNA-蛋白质相互作用
Analysis of RNA-protein interactions by flow cytometry.
作者信息
Brodsky Alexander S, Johnston Angus P, Trau Matt, Silver Pamela A
机构信息
Dana-Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 1 Jimmy Fund Way, SM 922, Boston, MA 02115, USA.
出版信息
Curr Opin Mol Ther. 2003 Jun;5(3):235-40.
Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.
流式细胞术与微珠编码技术的进步相结合，正逐渐发展成为一种强大的高通量分析分子相互作用的方法。该技术的应用包括抗体检测和单核苷酸多态性图谱分析。本文综述了用于分析RNA-蛋白质相互作用的微珠流式细胞术方法的最新进展，并讨论了新兴的微珠编码策略，这些策略将共同实现全基因组范围内RNA-蛋白质复合物的鉴定。微珠流式细胞术方法具有灵活性，为功能基因组学研究和小分子筛选提供了新的机遇。
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