Laboratory of Medical Genomics, Department of Human Genome Research, Kazusa DNA Research Institute, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
Electrophoresis. 2009 Dec;30(23):4008-19. doi: 10.1002/elps.200900211.
Advances in high-throughput screening, together with rapid progress in genomic and proteomic sciences, have considerably stimulated the development of a variety of biomarker discovery tools and have contributed to the improvement of clinical diagnosis. Major challenges include the obtaining of high-quality data sets based on assays that are rapid, reliable, and inexpensive, but still highly sensitive and easy to perform. The microsphere-based flow cytometric technology is a platform that is currently entering the diagnostic market. This article reviews the development and the applications of microsphere-based flow cytometric platforms in basic and applied proteomic research, and discusses bead-based assays used in clinical applications.
高通量筛选技术的进步，加上基因组学和蛋白质组学科学的快速发展，极大地刺激了各种生物标志物发现工具的发展，并有助于改善临床诊断。主要挑战包括基于快速、可靠和廉价但仍然高度敏感和易于执行的测定获得高质量数据集。基于微球的流式细胞术技术是一个目前正在进入诊断市场的平台。本文综述了基于微球的流式细胞术平台在基础和应用蛋白质组学研究中的发展和应用，并讨论了用于临床应用的基于珠的检测。
