Nudix水解酶超家族中一个对5-甲基-UTP（核糖-TTP）和UTP有活性的新亚家族。

A new subfamily of the Nudix hydrolase superfamily active on 5-methyl-UTP (ribo-TTP) and UTP.

作者信息

Xu WenLian, Shen JianYing, Dunn Christopher A, Bessman Maurice J

机构信息

Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA.

出版信息

J Biol Chem. 2003 Sep 26;278(39):37492-6. doi: 10.1074/jbc.M307639200. Epub 2003 Jul 18.

DOI:10.1074/jbc.M307639200

PMID:12871944

Abstract

A new subfamily of the Nudix hydrolases, identified by conserved amino acids upstream and downstream of the Nudix box, has been characterized. The cloned, expressed, and purified orthologous enzymes have major activities on the non-canonical nucleoside triphosphate 5-methyl-UTP (ribo-TTP) and the canonical nucleotide UTP. In addition to their homologous signature sequences and their similar substrate specificities, the members of the subfamily are inhabitants of or are related to the bacterial rhizosphere. We propose the acronym and mnemonic, utp, for the gene designating this unique UTPase.

摘要

通过Nudix框上游和下游保守氨基酸鉴定出的Nudix水解酶新亚家族已得到表征。克隆、表达和纯化的直系同源酶对非经典核苷三磷酸5-甲基-UTP（核糖-TTP）和经典核苷酸UTP具有主要活性。除了同源特征序列和相似的底物特异性外，该亚家族成员存在于细菌根际或与细菌根际相关。我们提议用首字母缩写词和助记符“utp”来命名这个独特的UTP酶基因。