O'Handley S F, Frick D N, Dunn C A, Bessman M J
Department of Biology and the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
J Biol Chem. 1998 Feb 6;273(6):3192-7. doi: 10.1074/jbc.273.6.3192.
orf186, a new member of the Nudix hydrolase family of genes, has been cloned and expressed, and the protein has been purified and identified as an enzyme highly specific for compounds of ADP. Its three major substrates are adenosine(5')triphospho(5')adenosine, ADP-ribose, and NADH, all implicated in a variety of cellular regulatory processes, supporting the notion that the function of the Nudix hydrolases is to monitor the concentrations of reactive nucleoside diphosphate derivatives and to help modulate their accumulation during cellular metabolism.
orf186是Nudix水解酶基因家族的一个新成员，已被克隆和表达，该蛋白质已被纯化，并被鉴定为一种对ADP化合物具有高度特异性的酶。它的三个主要底物是腺苷（5'）三磷酸（5'）腺苷、ADP-核糖和NADH，所有这些都参与了多种细胞调节过程，这支持了Nudix水解酶的功能是监测活性核苷二磷酸衍生物的浓度，并帮助调节它们在细胞代谢过程中的积累这一观点。
