Petti Nicola, Anghel Gabriel, Schimberni Mauro, De Rosa Luca, Mancini Stefano, Metro Giulio, Villaccio Barbara, Majolino Ignazio
Hematology and Bone Marrow Transplantation Unit, San Camillo-Forlanini Hospital, Rome, Italy.
Hematol J. 2003;4(4):285-8. doi: 10.1038/sj.thj.6200261.
We present the case of a 20-year-old man with acute lymphoblastic leukemia who received chemotherapy with vincristine, adriamycin, cyclophosphamide, methotrexate, teniposide (VM-26), and bleomicin, followed by an autologous bone marrow transplantation after total body irradiation (TBI)-cyclophosphamide-based conditioning regimen. At 14 years, despite the severe oligoasthenospermia, he fathered a healthy child by assisted reproductive technique (ART) consisting in controlled ovarian hyperstimulation of the patient's wife, transvaginal ovum pick up and microinjection of the ovum with a previously isolated sperm cell from the patient (intracytoplasmatic sperm injection, ICSI). As far as we know, that is the first documented case of successful paternity using microassisted fertilization with ICSI technique in a patient submitted to TBI-based bone marrow transplantation.
我们报告了一例20岁急性淋巴细胞白血病男性患者的病例，该患者接受了长春新碱、阿霉素、环磷酰胺、甲氨蝶呤、替尼泊苷（VM - 26）和博来霉素化疗，随后在基于全身照射（TBI）-环磷酰胺的预处理方案后进行了自体骨髓移植。14年后，尽管存在严重的少弱精子症，他通过辅助生殖技术（ART）使妻子受孕并诞下一名健康婴儿，该技术包括对患者妻子进行控制性卵巢过度刺激、经阴道取卵以及用先前从患者处分离的精子细胞对卵子进行显微注射（卵胞浆内单精子注射，ICSI）。据我们所知，这是首例有文献记载的在接受基于TBI的骨髓移植患者中使用ICSI技术进行微辅助受精成功孕育后代的病例。
