Sakaguchi S, Sakaguchi T, Nakamura I, Kudo Y
Department of Hygiene, St. Marianna University School of Medicine, Kanagawa.
Nihon Eiseigaku Zasshi. 1992 Dec;47(5):934-8. doi: 10.1265/jjh.47.934.
We studied changes of humoral immunity, such as complement pathway activity, C3 contents and contents of immunoglobulin, in mice injected subcutaneously with BeCl2 or CuCl2 once a week for 12 weeks. Mean body weights of JCL: ICR female mice were approximately 30g in control mice (control group; n = 7), in mice injected with Be (Be group; n = 8) and in mice injected with Cu (Cu group; n = 8). Values of classical complement pathway activity (CH50) were 18.8 +/- 1.4 U per ml, 15.3 +/- 1.8 U per ml and 16.7 +/- 1.3 U per ml in the control group, Be group and Cu group, respectively. The CH50 values of Be and Cu groups were significantly lower than that of the control group (P < 0.01). In contrast, values of alternative complement pathway activity (ACH50) and contents of C3 were almost constant in the three groups. The immunoglobulin content in the Be group tended to increase. The activity of alanine aminotransferase in the Be group was markedly higher than that in the control group (P < 0.05), and the aspartate aminotransferase activity was also high. The CH50 value of mice injected with a small amount of Be once a week over a 12-week period decreased markedly, although either the ACH50 value or C3 content was the same as in the control group. The immunoglobulin content somewhat increased in the Be group. These results suggest the possibility that immune complex is induced by Be.
我们研究了每周皮下注射一次BeCl2或CuCl2，持续12周的小鼠体液免疫变化，如补体途径活性、C3含量和免疫球蛋白含量。JCL: ICR雌性小鼠在对照组（对照组；n = 7）、注射Be的小鼠（Be组；n = 8）和注射Cu的小鼠（Cu组；n = 8）中的平均体重约为30g。经典补体途径活性（CH50）值在对照组、Be组和Cu组中分别为每毫升18.8±1.4 U、15.3±1.8 U和16.7±1.3 U。Be组和Cu组的CH50值显著低于对照组（P < 0.01）。相比之下，替代补体途径活性（ACH50）值和C3含量在三组中几乎保持恒定。Be组的免疫球蛋白含量有增加趋势。Be组的丙氨酸转氨酶活性显著高于对照组（P < 0.05），天冬氨酸转氨酶活性也较高。在12周内每周注射少量Be的小鼠，其CH50值显著下降，尽管ACH50值或C3含量与对照组相同。Be组的免疫球蛋白含量有所增加。这些结果提示Be可能诱导免疫复合物形成。
