枸橼酸盐-磷酸盐-葡萄糖（CPD）保存血液中的补体。

Complement in CPD-stored blood.

作者信息

Schifferli J A, Boralessa H, Howarth H L, Whitwam J G, Rees A J

出版信息

Vox Sang. 1982;42(4):198-202. doi: 10.1111/j.1423-0410.1982.tb01095.x.

DOI:10.1111/j.1423-0410.1982.tb01095.x

Abstract

We studied complement functions, breakdown of C3 and aggregation of immunoglobulins in whole blood stored in citrate phosphate dextrose at 4 degrees C for up to 3 weeks from 10 normal blood donors. No significant changes in total haemolytic complement (CH50) and alternative pathway haemolytic complement activity were detected. However, the complement-mediated capacity of the sera to solubilise a pre-formed immune precipitate decreased significantly at 2 and 3 weeks (p less than 0.02 at 2 weeks, p less than 0.003 at 3 weeks). No C3 conversion could be detected by immunoelectrophoresis but alteration in C3 was attested by a significant increase in breakdown products of C3 by 1 week (p less than 0.002) and all were above the normal range after 3 weeks (p less than 0.0001). No aggregation of immunoglobulins could be detected using two immune complex assays.

摘要

我们对10名正常献血者在4℃下于枸橼酸盐-磷酸盐-葡萄糖溶液中储存长达3周的全血中的补体功能、C3分解及免疫球蛋白聚集情况进行了研究。未检测到总溶血补体（CH50）和替代途径溶血补体活性有显著变化。然而，血清的补体介导溶解预先形成的免疫沉淀物的能力在第2周和第3周时显著下降（第2周时p<0.02，第3周时p<0.003）。免疫电泳未检测到C3转化，但C3的改变通过C3分解产物在第1周时显著增加得到证实（p<0.002），且在3周后所有指标均高于正常范围（p<0.0001）。使用两种免疫复合物检测方法均未检测到免疫球蛋白聚集。