植物毒素蓖麻毒素和相思豆毒素从不同质膜位点的细胞内运输。

Intracellular transport of plant toxins ricin and viscumin from different plasma membrane sites.

作者信息

Moisenovich Mihail, Agapov Igor, Marx Uwe, Bereiter-Hahn Jürgen, Tonevitsky Alexander

机构信息

Department of Cell Physiology and Immunology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia.

出版信息

Arzneimittelforschung. 2003;53(6):470-5. doi: 10.1055/s-0031-1297135.

DOI:10.1055/s-0031-1297135

PMID:12872616

Abstract

Binding of ricin and viscumin to paraformaldehyde fixed cell surface has been studied by confocal laser scanning microscopy (CLSM). Both toxins were labeled with different fluorochromes to allow for their identification after being applied jointly. The experiments indicated that viscumin and ricin bind to different cell receptors. Viscumin bound to the very periphery of the cells including lamellapodia and cell contact regions. Labeled ricin became localized in surface clusters located close to the cell body. The binding of toxins to the cell membrane was completely inhibited by 100 mmol/l lactose and in the presence of unlabeled homological toxins 500 times in abundance of the fluorochromed toxins. The experiments indicate that uptake and intracellular transport of ricin and viscumin starts from different membrane sites.

摘要

通过共聚焦激光扫描显微镜（CLSM）研究了蓖麻毒素和相思子毒素与多聚甲醛固定的细胞表面的结合情况。两种毒素都用不同的荧光染料标记，以便在联合应用后进行识别。实验表明，相思子毒素和蓖麻毒素与不同的细胞受体结合。相思子毒素结合在细胞的最外围，包括片状伪足和细胞接触区域。标记的蓖麻毒素定位于靠近细胞体的表面簇中。100 mmol/l乳糖以及在未标记的同源毒素存在且其数量是荧光染料标记毒素500倍的情况下，毒素与细胞膜的结合被完全抑制。实验表明，蓖麻毒素和相思子毒素的摄取和细胞内转运起始于不同的膜位点。