Kishimoto T, Fukuzawa Y, Abe M, Hashimoto M, Ohno M, Tada M
Department of Environmental Medicine, Shimane Medical University, Izumo.
Nihon Eiseigaku Zasshi. 1992 Dec;47(5):965-70. doi: 10.1265/jjh.47.965.
The effect of copper sulfate (CuSO4) on cultured human vascular endothelial (HVE) cells and cultured human fibroblasts (HAIN-55) was investigated. HVE cells were collected from umbilical veins by enzymatic digestion with collagenase. The viability, subsequent growth and DNA synthesis of both cell types were inhibited concentration-dependently by the addition of copper. The cytotoxic effect of copper on the morphology of these cells was also concentration-dependent. However, the cytotoxic effect of copper on the viability, subsequent growth and DNA synthesis was greater in HVE cells than in HAIN-55 cells. These results suggest that HVE cells are more susceptible to concentration-dependent copper cytotoxicity than HAIN-55 cells are, and that copper could induce vascular endothelial injury, which may be involved in the pathogenesis of cardiovascular disease.
研究了硫酸铜（CuSO4）对培养的人血管内皮（HVE）细胞和培养的人成纤维细胞（HAIN-55）的影响。通过用胶原酶酶消化从脐静脉收集HVE细胞。添加铜后，两种细胞类型的活力、后续生长和DNA合成均呈浓度依赖性抑制。铜对这些细胞形态的细胞毒性作用也呈浓度依赖性。然而，铜对活力、后续生长和DNA合成的细胞毒性作用在HVE细胞中比在HAIN-55细胞中更大。这些结果表明，HVE细胞比HAIN-55细胞对浓度依赖性铜细胞毒性更敏感，并且铜可诱导血管内皮损伤，这可能参与心血管疾病的发病机制。
