Tada M, Okazaki M, Kishimoto T, Fukuzawa Y, Isobe M
Department of Environmental Medicine I, Shimane Medical University, Izumo, Japan.
Hum Cell. 1988 Jun;1(2):198-206.
The effects of antifebriles and anticancer drug on human vascular endothelial cells (HVE) and several cultured human cells were investigated. The HVE were isolated from umbilical cord veins by enzyme treatment and cultured successively in aerated synthetic medium, RPMI-1640, with 20% preclostrum new born calf serum. The presence of factor VIII antigen in the HVE was determined by enzyme-labeled antibody method. Cell count and protein amount were examined at regular intervals. At 3 hour-expose, sulpyrine was more toxic to the cultured cells than aspirin at 37 degrees C. The cytotoxicity of sulpyrine was markedly enhanced at 40 degrees C than at 37 degrees C. However, there was no enhancement in the cytotoxicity of aspirin at 40 degrees C. Cultured HVE and normal human fetal lung (HAIN-55) cells at 37 degrees C were sensitive to sulpyrine, and their sensitivity of the cells to the drug were markedly enhanced when they were incubated at 41 degrees C. In contrast, sensitivity of malignant human cells (HeLa cells) to sulpyrine was not found at 37 degrees C, however sensitivity of the cells to the drug was manifested at 41 degrees C of incubation. There was no effect of 5-fluorouracil (FU) on the growth of HVE and HAIN-55 cells at 41 degrees C, while HeLa cells showed high susceptibility to FU at the same temperature. The results showed the possibility that normal human cells may be sensitive to antifebrile drugs but not to anticancer drug at ordinary and high temperature, whereas malignant human cell may be susceptible to both antifebrile drugs and anticancer drug at high temperature.
研究了退热药和抗癌药对人血管内皮细胞（HVE）及几种培养的人细胞的作用。通过酶处理从脐带静脉中分离出HVE，并在含有20%初乳新生小牛血清的充气合成培养基RPMI - 1640中连续培养。采用酶标抗体法测定HVE中因子VIII抗原的存在情况。定期检查细胞计数和蛋白含量。在37℃下暴露3小时，舒林酸对培养细胞的毒性比阿司匹林大。40℃时舒林酸的细胞毒性比37℃时明显增强。然而，40℃时阿司匹林的细胞毒性没有增强。37℃下培养的HVE和正常人胎儿肺（HAIN - 55）细胞对舒林酸敏感，当在41℃孵育时，它们对该药物的敏感性明显增强。相反，恶性人细胞（HeLa细胞）在37℃时对舒林酸不敏感，但在41℃孵育时对该药物敏感。在41℃时，5 - 氟尿嘧啶（FU）对HVE和HAIN - 55细胞的生长没有影响，而HeLa细胞在相同温度下对FU高度敏感。结果表明，正常人细胞在常温及高温下可能对退热药敏感但对抗癌药不敏感，而恶性人细胞在高温下可能对退热药和抗癌药都敏感。
