Fujimura A, Shiga T, Sudoh T, Ohashi K, Ebihara A
Department of Clinical Pharmacology, Jichi Medical School, Tochigi, Japan.
Jpn J Pharmacol. 1992 Dec;60(4):323-6. doi: 10.1254/jjp.60.323.
Daily variation in the effects of furosemide, a loop diuretic agent, was examined in Wistar rats maintained under conditions of light from 7 a.m. to 7 p.m. and dark from 7 p.m. to 7 a.m. Furosemide (30 mg/kg) was given orally at 12 p.m., 4 a.m., 8 a.m., 12 a.m., 4 p.m. or 8 p.m. Urine was collected for 8 hr after furosemide administration, and urinary excretions of sodium and furosemide were determined. There were significant daily variations in the urine volume and urinary excretions of sodium and furosemide with a peak at 8 a.m. and a trough at 12 p.m. Significant correlations were observed between the urinary amount of furosemide and its diuretic effects (urine volume and urinary sodium excretion). These results suggest that the diuretic effects of furosemide show daily variations which are, at least in part, caused by the daily variation in the urinary excretion of furosemide.
在上午7点至晚上7点光照、晚上7点至上午7点黑暗的条件下饲养的Wistar大鼠中，研究了袢利尿剂呋塞米作用的日变化。在中午12点、凌晨4点、上午8点、午夜12点、下午4点或晚上8点口服呋塞米（30毫克/千克）。给药后收集8小时尿液，测定钠和呋塞米的尿排泄量。尿量以及钠和呋塞米的尿排泄量存在显著的日变化，上午8点达到峰值，中午12点达到谷值。呋塞米的尿量与其利尿作用（尿量和尿钠排泄）之间存在显著相关性。这些结果表明，呋塞米的利尿作用存在日变化，至少部分是由呋塞米尿排泄的日变化引起的。
