Fujimura A, Sudoh T, Ebihara A
Department of Clinical Pharmacology, Jichi Medical School, Tochigi, Japan.
Chronobiol Int. 1994 Apr;11(2):113-8. doi: 10.3109/07420529409055897.
The influence of age on the time-dependent difference in urinary excretion of furosemide, a loop diuretic agent, was examined in this longitudinal study. Male Wistar rats were maintained under conditions of light from 07:00 to 19:00 h and dark from 19:00 to 07:00 h. Furosemide (30 mg/kg) was given orally at 12:00 h (day trial) or 00:00 h (night trial) to rats at 3 months of age, and urine was collected for 8 h after dosage. Thereafter, the identical protocol was repeated using the same animals at 6, 9, 12, 15, 18, and 21 months of age. The urinary excretion of furosemide was significantly greater in the day than in the night trial at 3 months of age. Such a time-dependent difference was observed for up to 15 months, but disappeared at 18 and 21 months of age. The time-dependent difference in urinary excretion of furosemide (day trial - night trial) decreased gradually throughout the observation period of the study. These results suggest that the time-dependent difference in the urinary excretion of furosemide diminishes during the aging process and disappears by 18 months of age in male Wistar rats.
在这项纵向研究中，研究了年龄对袢利尿剂呋塞米尿排泄时间依赖性差异的影响。雄性Wistar大鼠饲养在光照时间为07:00至19:00 h、黑暗时间为19:00至07:00 h的条件下。在3月龄大鼠的12:00时（日间试验）或00:00时（夜间试验）口服给予呋塞米（30 mg/kg），给药后收集8小时尿液。此后，在6、9、12、15、18和21月龄时，使用相同的动物重复相同的实验方案。在3月龄时，呋塞米的尿排泄量在日间试验中显著高于夜间试验。这种时间依赖性差异在15月龄时均可观察到，但在18和21月龄时消失。在整个研究观察期内，呋塞米尿排泄的时间依赖性差异（日间试验-夜间试验）逐渐减小。这些结果表明，呋塞米尿排泄的时间依赖性差异在衰老过程中逐渐减小，并在雄性Wistar大鼠18月龄时消失。
