通过辐射杂种图谱和连锁分析证明猪7号染色体与人类6号、14号和15号染色体之间的同线性保守性。

Conservation of the syntenies between porcine chromosome 7 and human chromosomes 6, 14 and 15 demonstrated by radiation hybrid mapping and linkage analysis.

作者信息

Tanaka M, Matsumoto T, Yanai S, Domukai M, Toki D, Hayashi T, Kiuchi S, Yasue H, Uenishi H, Kobayashi E, Awata T

机构信息

STAFF Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan.

出版信息

Anim Genet. 2003 Aug;34(4):255-63. doi: 10.1046/j.1365-2052.2003.00999.x.

DOI:10.1046/j.1365-2052.2003.00999.x

PMID:12873213

Abstract

Comparative mapping studies facilitate the identification of genes located in quantitative trait locus (QTL) regions in domestic animals by utilizing information from the human genome. Radiation hybrid (RH) mapping is effective for this purpose because of its high resolution in ordered gene mapping on chromosomes. We constructed an RH map of pig chromosome 7, by adding 23 markers associated with genes. This RH map clearly demonstrated the mosaic of homology between pig chromosome 7 (SSC7) and human chromosomes 6, 14 and 15 at a 'gene' level, and was confirmed by linkage analysis. Clarification of the homology of SSC7 to human chromosomes will contribute to the elucidation of the gene(s) responsible for QTL detected on this chromosome.

摘要

比较图谱研究通过利用人类基因组信息来促进家畜数量性状基因座（QTL）区域中基因的鉴定。辐射杂种（RH）图谱绘制对于此目的很有效，因为它在染色体上有序基因图谱绘制方面具有高分辨率。我们通过添加23个与基因相关的标记构建了猪7号染色体的RH图谱。该RH图谱在“基因”水平上清楚地展示了猪7号染色体（SSC7）与人类6号、14号和15号染色体之间的同源镶嵌情况，并通过连锁分析得到了证实。阐明SSC7与人类染色体的同源性将有助于阐明该染色体上检测到的QTL所对应的基因。