Zhang Jinghu, Xiong Yuanzhu, Zuo Bo, Lei Minggang, Jiang Siwen, Li Feng'e, Zheng Rong, Li Jialian
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
J Genet Genomics. 2007 Jan;34(1):10-6. doi: 10.1016/S1673-8527(07)60002-2.
The use of markers and linkage map construction are important for QTL mapping in pigs. In this article, the genetic characteristics were studied and the linkage map was constructed in a pig resource population including 214 individuals by typing 39 microsatellite marker loci on Sus scrofa chromosomes, SSC4, SSC6, SSC7, SSC8, and SSC13. Results indicated that the average allele number, the average observed heterozygosity (H(0)), and the average polymorphism information content (PIC) in F(1) and F(2) population were 3.2, 0.528, 0.463 and 3.2, 0.496, 0.447, respectively. In the pig resource population, the average informative meiosis (IM) was 217.4 (44-316), and the average linkage map length between the two sexes on the five chromosomes were 172.3 cM (SSC4), 168.7 cM (SSC6), 191.7 cM (SSC7), 197.3 cM (SSC8), and 178.3 cM (SSC13). The orders of microsatellite marker loci in the linkage maps were identical to, but the length was greater than, those of USDA-MARC reference map. The results of this research showed the genetic relationship and genetic characteristics of the microsatellite markers in the pig resource family population, and the linkage map could be used to for QTL mapping in the subsequent study.
标记的使用和连锁图谱构建对于猪的数量性状基因座（QTL）定位至关重要。在本文中，通过对野猪（Sus scrofa）4号、6号、7号、8号和13号染色体上的39个微卫星标记位点进行分型，研究了一个包含214个个体的猪资源群体的遗传特征并构建了连锁图谱。结果表明，F1和F2群体中的平均等位基因数、平均观察杂合度（H(0)）和平均多态信息含量（PIC）分别为3.2、0.528、0.463和3.2、0.496、0.447。在该猪资源群体中，平均有效减数分裂（IM）为217.4（44 - 316），五条染色体上两性之间的平均连锁图谱长度分别为172.3 cM（4号染色体）、168.7 cM（6号染色体）、191.7 cM（7号染色体）、197.3 cM（8号染色体）和178.3 cM（13号染色体）。连锁图谱中微卫星标记位点的顺序与美国农业部猪基因组研究协作组（USDA - MARC）参考图谱相同，但长度更长。本研究结果显示了猪资源家系群体中微卫星标记的遗传关系和遗传特征，该连锁图谱可用于后续研究中的QTL定位。
