Maksimova N L, Blazhevich O V, Fomichev Iu K
Mikrobiologiia. 1992 Sep-Oct;61(5):818-23.
The contemporary data of the participation of phenylalanine in the biosynthesis of fluorescent pigment pyoverdine PM of Pseudomonas putida strain M are presented. Using aro1phu1 mutant of this strain, it has been shown that one of the precursors of the dihydroxyquinoline moiety of the pyoverdine PM is phenylalanine in the D- or L-form. These results were confirmed in experiments with 14C-phenylalanine incorporation. Pyoverdine PM that was synthesized by aro1phu1 mutant from exogenous phenylalanine is identical with the pigment from wild type cells.
本文展示了苯丙氨酸参与恶臭假单胞菌M菌株荧光色素绿脓菌素PM生物合成的当代数据。利用该菌株的aro1phu1突变体,已表明绿脓菌素PM二羟基喹啉部分的前体之一是D型或L型苯丙氨酸。这些结果在14C-苯丙氨酸掺入实验中得到了证实。aro1phu1突变体从外源苯丙氨酸合成的绿脓菌素PM与野生型细胞的色素相同。