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[使用流式细胞荧光术对异步和同步酵母培养物的研究]

[Study of asynchronous and synchronous yeast cultures using flow cytofluorometry].

作者信息

Ivanov V N, Stabnikova E V

出版信息

Mikrobiologiia. 1992 Sep-Oct;61(5):887-92.

PMID:1287409
Abstract

Distribution of Saccharomyces cerevisiae, Candida boidinii and Candida tropicalis cells according to DNA content was investigated using laser flow cytofluorometry. Cells distribution curves according to DNA content possessed two maxima in case the sample belonged to the exponential phase of the asynchronous batch culture, or one maximum in case the sample was from the stationary phase of growth. In synchronous cultures variations of cells distribution curves according to DNA content (age structure of the population) were demonstrated and the curves with one maximum and plateau were observed.

摘要

使用激光流式细胞荧光测定法研究了酿酒酵母、博伊丁假丝酵母和热带假丝酵母细胞根据DNA含量的分布情况。如果样品属于异步分批培养的指数期,则根据DNA含量的细胞分布曲线有两个最大值;如果样品来自生长的稳定期,则有一个最大值。在同步培养中,证明了根据DNA含量的细胞分布曲线(群体的年龄结构)的变化,并观察到具有一个最大值和平原的曲线。

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