Suzuki T, Rogers A L, Magee P T
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Yeast. 1986 Mar;2(1):53-8. doi: 10.1002/yea.320020104.
Hybridization was shown to occur both between strains of the imperfect diploid yeast Candida albicans and between C. albicans and the distantly related haploid yeast Candida (Pichia) guilliermondii. Prototrophic hybrids were selected from crosses of multiply marked auxotrophic mutants of the two species. In most cases, mild ultraviolet irradiation of the C. albicans partner was required. Examination of auxotrophic markers in segregants from the hybrids indicated that recombination, rather than heterokaryon formation, had occurred in these crosses. The DNA content of the hybrids varied from diploid or aneuploid (for crosses between C. albicans and C. guilliermondii) to triploid (for C. albicans x C. albicans). It seems possible that genetic exchange analogous to this may occur in nature.
杂交现象在不完全二倍体酵母白色念珠菌的菌株之间以及白色念珠菌与远缘单倍体酵母季也蒙念珠菌（毕赤酵母属）之间均有发生。从这两个物种的多重标记营养缺陷型突变体杂交后代中筛选出了原养型杂种。在大多数情况下，需要对白色念珠菌亲本进行轻度紫外线照射。对杂种分离株中营养缺陷型标记的检测表明，这些杂交中发生了重组而非异核体形成。杂种的DNA含量从二倍体或非整倍体（白色念珠菌与季也蒙念珠菌杂交）到三倍体（白色念珠菌×白色念珠菌）不等。类似这样的基因交换在自然界中似乎有可能发生。
