Wen Duan-Cheng, Yang Cai-Xia, Cheng Yong, Li Jin-Song, Liu Zhong-Hua, Sun Qing-Yuan, Zhang Jia-Xin, Lei Lei, Wu Yu-Qi, Kou Zhao-Hui, Chen Da-Yuan
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Mol Reprod Dev. 2003 Sep;66(1):38-45. doi: 10.1002/mrd.10333.
Interspecies nuclear transfer is an invaluable tool for studying nucleus-cytoplasm interactions; and at the same time, it provides a possible alternative to clone animals whose oocytes are difficult to obtain. In the present study, we investigated the possibility of cloning cat embryos using rabbit oocytes, and compared the developmental capacity; the timing of embryogenesis of the cat-rabbit cloned embryos with that of the cat-cat or the rabbit-rabbit cloned embryos. When cultured in M199, the rate of blastocyst formation of the cat-rabbit embryos was 6.9%, which was not significantly different than that of the cat-cat embryos (10.5%). However, the rate of blastocyst formation of rabbit-rabbit embryos (22.9%) was significantly greater than that of both the cat-rabbit and the cat-cat embryos (P < 0.05). The timing of the first three cleavages for the cat-rabbit embryos was similar to that of the rabbit-rabbit embryos, but significantly faster than that of the cat-cat embryos (P < 0.05), while the time to form blastocysts was similar to that of cat-cat embryos, but significantly slower than that of the rabbit-rabbit embryos (P < 0.05). Both M199 and SOF medium were evaluated for culturing cat-rabbit embryos; the rate of blastocyst formation in SOF (14.5%) was significantly greater than that in M199 (6.9%) (P < 0.05). These results demonstrate that: (1) the cat-rabbit embryos possess equal developmental capacity as cat-cat embryos; (2) the timing of the first three cleavages for the cat-rabbit embryos is recipient-specific, while the time to form blastocysts is donor nucleus-specific; and (3) SOF medium may be beneficial to overcome the morula-to-blastocyst block for cat-rabbit cloned embryos.
种间核移植是研究核质相互作用的一种宝贵工具；同时，它为克隆那些难以获得卵母细胞的动物提供了一种可能的替代方法。在本研究中，我们研究了使用兔卵母细胞克隆猫胚胎的可能性，并比较了其发育能力；猫 - 兔克隆胚胎与猫 - 猫或兔 - 兔克隆胚胎的胚胎发生时间。当在M199中培养时，猫 - 兔胚胎的囊胚形成率为6.9%，与猫 - 猫胚胎（10.5%）无显著差异。然而，兔 - 兔胚胎的囊胚形成率（22.9%）显著高于猫 - 兔和猫 - 猫胚胎（P < 0.05）。猫 - 兔胚胎的前三次卵裂时间与兔 - 兔胚胎相似，但明显快于猫 - 猫胚胎（P < 0.05），而形成囊胚的时间与猫 - 猫胚胎相似，但明显慢于兔 - 兔胚胎（P < 0.05）。我们评估了M199和SOF培养基对猫 - 兔胚胎的培养效果；SOF培养基中的囊胚形成率（14.5%）显著高于M199培养基（6.9%）（P < 0.05）。这些结果表明：（1）猫 - 兔胚胎具有与猫 - 猫胚胎同等的发育能力；（2）猫 - 兔胚胎的前三次卵裂时间是受体特异性的，而形成囊胚的时间是供体核特异性的；（3）SOF培养基可能有助于克服猫 - 兔克隆胚胎的桑椹胚到囊胚的阻滞。
