Ahn Chi-Yong, Park Myung-Hwan, Joung Seung-Hyun, Kim Hee-Sik, Jang Kam-Yong, Oh Hee-Mock
Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea.
Environ Sci Technol. 2003 Jul 1;37(13):3031-7. doi: 10.1021/es034048z.
The growth of Microcystis aeruginosa UTEX 2388 was repressed by ultrasonic radiation and resulted in an increased chlorophyll a content and cell size, suggesting the inhibition of cell division. However, growth was recovered immediately after the interruption of ultrasonication. In addition to the disruption of gas vesicles, other mechanisms of growth inhibition were also investigated. Although free radicals were produced by ultrasonication and hydrogen peroxide, the resulting lipid peroxidation in the cells was not comparable, indicating minimal damage by the free radicals. Ultrasonic radiation late in the day was found to be most effective in reducing the growth rate of M. aeruginosa, and this timing also corresponded to the phase of daily cell division. In an enclosure experiment, ultrasonic radiation reduced the pH, DO, total nitrogen, and total phosphorus, whereas it increased the water temperature, conductivity, and orthophosphate concentration. The algal cell density and chlorophyll a concentration drastically decreased after 3 d of ultrasonication, plus the cyanobacterial proportion was selectively reduced as compared to other algal species. Accordingly, ultrasonic radiation would appear to have considerable potential as an effective control method for cyanobacterial blooms.
铜绿微囊藻UTEX 2388的生长受到超声辐射的抑制，导致叶绿素a含量增加和细胞大小增大，这表明细胞分裂受到抑制。然而，超声处理中断后，生长立即恢复。除了气荚膜的破坏外，还研究了其他生长抑制机制。尽管超声处理和过氧化氢会产生自由基，但细胞中由此产生的脂质过氧化作用并不相当，表明自由基造成的损伤最小。发现傍晚的超声辐射对降低铜绿微囊藻的生长速率最有效，而且这个时间也与每日细胞分裂阶段相对应。在围隔实验中，超声辐射降低了pH值、溶解氧、总氮和总磷，而增加了水温、电导率和正磷酸盐浓度。超声处理3天后，藻类细胞密度和叶绿素a浓度急剧下降，而且与其他藻类相比，蓝藻比例被选择性降低。因此，超声辐射似乎有很大潜力成为一种有效的蓝藻水华控制方法。
