Liu Ping, Wang Yuan-Fang, Ewis Hosam E, Abdelal Ahmed, Lu Chung Dar, Weber Irene T
Biology Department, Georgia State University, Atlanta, GA, USA.
Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1472-3. doi: 10.1107/s0907444903011405. Epub 2003 Jul 23.
Crystals have been grown of the carboxylesterase Est30 from Bacillus stearothermophilus by hanging-drop vapor diffusion using ammonium sulfate as precipitant. The crystals diffracted to better than 2.0 A resolution. X-ray diffraction data were reduced in space group C222(1), with unit-cell parameters a = 55.83, b = 58.15, c = 179.65 A. R(merge) was 0.038 for 17 449 independent reflections with a completeness of 85.1%. V(M) was calculated to be 2.43 A(3) Da(-1), which suggested that there was one molecule of Est30 in the asymmetric unit. These crystals are suitable for structure determination.
通过使用硫酸铵作为沉淀剂的悬滴气相扩散法，已培养出嗜热脂肪芽孢杆菌羧酸酯酶Est30的晶体。这些晶体的衍射分辨率优于2.0 Å。X射线衍射数据在空间群C222(1)中进行处理，晶胞参数为a = 55.83、b = 58.15、c = 179.65 Å。对于17449个独立反射，R(merge)为0.038，完整性为85.1%。计算出的V(M)为2.43 ų Da⁻¹，这表明不对称单元中有一个Est30分子。这些晶体适合用于结构测定。
