Strancar J, Schara M, Pecar S
Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
J Membr Biol. 2003 May 1;193(1):15-22. doi: 10.1007/s00232-002-2003-7.
An electron paramagnetic resonance (EPR)-based membrane surface characterization method is presented to detect the properties of the carbohydrate-rich part of membrane surfaces as well as carbohydrate interaction with other membrane constituents and water-soluble molecules. The proposed method relies on the spin-labeling and spectral decomposition based on spectral simulation and optimization with EPRSIM software. In order to increase the sensitivity of characterization to the carbohydrate-rich part of the membrane surface, the sucrose-contrasting approach is introduced. With this method, which was established on model membranes with glycolipids and tested on erythrocyte membrane, we were able to characterize the surface and lipid bilayer lateral heterogeneity. Additionally, some properties of the interaction between glycocalyx and lipid bilayer as well as between glycocalyx and sucrose molecules were determined. The experiments also provided some information about the anchoring and aggregation of the glycosylated molecules. According to the results, some functions of the glycosylated surface are discussed.
本文提出了一种基于电子顺磁共振（EPR）的膜表面表征方法，用于检测膜表面富含碳水化合物部分的性质，以及碳水化合物与其他膜成分和水溶性分子的相互作用。该方法基于自旋标记以及基于EPRSIM软件的光谱模拟和优化进行光谱分解。为了提高对膜表面富含碳水化合物部分的表征灵敏度，引入了蔗糖对比法。通过在含有糖脂的模型膜上建立并在红细胞膜上进行测试的该方法，我们能够表征表面和脂质双层的横向异质性。此外，还确定了糖萼与脂质双层之间以及糖萼与蔗糖分子之间相互作用的一些性质。实验还提供了一些关于糖基化分子的锚定和聚集的信息。根据结果，讨论了糖基化表面的一些功能。
