利用脂质体和电子顺磁共振自旋标记研究生物膜中的脂质组织。
Studying lipid organization in biological membranes using liposomes and EPR spin labeling.
作者信息
Subczynski Witold K, Raguz Marija, Widomska Justyna
机构信息
Department of Biophysics, Medical College of Wisconsin, Milwaukee, USA.
出版信息
Methods Mol Biol. 2010;606:247-69. doi: 10.1007/978-1-60761-447-0_18.
Electron paramagnetic resonance (EPR) spin-labeling methods provide a unique opportunity to determine the lateral organization of lipid bilayer membranes by discrimination of coexisting membrane domains or coexisting membrane phases. In some cases, coexisting membrane domains can be characterized without the need for their physical separation by profiles of alkyl chain order, fluidity, hydrophobicity, and oxygen diffusion-concentration product in situ. This chapter briefly explains how EPR spin-labeling methods can be used to obtain the above-mentioned profiles across lipid bilayer membranes (liposomes). These procedures will be illustrated by EPR measurements performed on multilamellar liposomes made of lipid extracts from cortical and nuclear fractions of the fiber cell plasma membrane of a cow-eye lens. To better elucidate the major factors that determine membrane properties, results for eye lens lipid membranes and simple model membranes that resemble the basic lipid composition of biological membranes will be compared.
电子顺磁共振（EPR）自旋标记方法为通过区分共存的膜结构域或共存的膜相来确定脂质双分子层膜的侧向组织提供了独特的机会。在某些情况下，无需对共存的膜结构域进行物理分离，就可以通过原位的烷基链有序性、流动性、疏水性和氧扩散 - 浓度积曲线来表征它们。本章简要解释了如何使用EPR自旋标记方法来获取跨脂质双分子层膜（脂质体）的上述曲线。这些程序将通过对由牛眼晶状体纤维细胞质膜的皮质和核部分的脂质提取物制成的多层脂质体进行的EPR测量来说明。为了更好地阐明决定膜性质的主要因素，将比较眼晶状体脂质膜和类似于生物膜基本脂质组成的简单模型膜的结果。
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