Cui Shuya, Hu Xiaoli, Chen Xingguo, Hu Zhide
National Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, 730000 Lanzhou, P.R. China.
Anal Bioanal Chem. 2003 Sep;377(2):370-4. doi: 10.1007/s00216-003-2045-4. Epub 2003 Jul 19.
A capillary zone electrophoresis (CZE) method has been developed for simultaneous assay of two bioactive components (p-tyrosol and salidroside) in Rhodiola crenulata and Rhodiola kirilowii for the first time. Those analytes were successfully separated within 15 min using 50 mmol L(-1) (pH 9.62) borate containing 30% methanol as running buffer. Regression equations revealed linear relationships (correlation coefficients 0.9998-0.9999) between peak area and concentration of the two analytes. The relative standard deviations ( RSD) of the migration times and the peak areas of two constituents ranged from 0.51 to 0.57% and from 0.65 to 1.17%, respectively, intra-day, and from 4.91 to 6.93% and from 3.51 to 5.33%, respectively, inter-day. The recoveries of two constituents ranged from 96.24 to 103.15%.
首次建立了一种毛细管区带电泳（CZE）方法，用于同时测定大花红景天和狭叶红景天中的两种生物活性成分（对酪醇和红景天苷）。使用含30%甲醇的50 mmol L⁻¹（pH 9.62）硼酸盐作为运行缓冲液，在15分钟内成功分离了这些分析物。回归方程显示两种分析物的峰面积与浓度之间呈线性关系（相关系数为0.9998 - 0.9999）。两种成分迁移时间和峰面积的日内相对标准偏差（RSD）分别为0.51%至- 0.57%和0.65%至1.17%，日间分别为4.91%至6.93%和3.51%至5.33%。两种成分的回收率为96.24%至103.15%。
