采用毛细管区带电泳法测定三种大花红景天样品和一种狭叶红景天样品中的对酪醇和红景天苷含量。
Determination of p-tyrosol and salidroside in three samples of Rhodiola crenulata and one of Rhodiola kirilowii by capillary zone electrophoresis.
作者信息
Cui Shuya, Hu Xiaoli, Chen Xingguo, Hu Zhide
机构信息
National Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, 730000 Lanzhou, P.R. China.
出版信息
Anal Bioanal Chem. 2003 Sep;377(2):370-4. doi: 10.1007/s00216-003-2045-4. Epub 2003 Jul 19.
A capillary zone electrophoresis (CZE) method has been developed for simultaneous assay of two bioactive components (p-tyrosol and salidroside) in Rhodiola crenulata and Rhodiola kirilowii for the first time. Those analytes were successfully separated within 15 min using 50 mmol L(-1) (pH 9.62) borate containing 30% methanol as running buffer. Regression equations revealed linear relationships (correlation coefficients 0.9998-0.9999) between peak area and concentration of the two analytes. The relative standard deviations ( RSD) of the migration times and the peak areas of two constituents ranged from 0.51 to 0.57% and from 0.65 to 1.17%, respectively, intra-day, and from 4.91 to 6.93% and from 3.51 to 5.33%, respectively, inter-day. The recoveries of two constituents ranged from 96.24 to 103.15%.
首次建立了一种毛细管区带电泳（CZE）方法，用于同时测定大花红景天和狭叶红景天中的两种生物活性成分（对酪醇和红景天苷）。使用含30%甲醇的50 mmol L⁻¹（pH 9.62）硼酸盐作为运行缓冲液，在15分钟内成功分离了这些分析物。回归方程显示两种分析物的峰面积与浓度之间呈线性关系（相关系数为0.9998 - 0.9999）。两种成分迁移时间和峰面积的日内相对标准偏差（RSD）分别为0.51%至- 0.57%和0.65%至1.17%，日间分别为4.91%至6.93%和3.51%至5.33%。两种成分的回收率为96.24%至103.15%。
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