Linh P T, Kim Y H, Hong S P, Jian J J, Kang J S
College of Pharmacy, Chungnam National University, Taejon, Korea.
Arch Pharm Res. 2000 Aug;23(4):349-52. doi: 10.1007/BF02975446.
A reversed-phase high performance liquid chromatographic method was developed to determine salidroside and tyrosol simultaneously in the Rhodiola rosea. The optimum condition was Nova-Pak C18 as stationary phase, 6.5% methanol in water as mobile phase and detection at UV 225 nm. The identification was carried out by comparing the retention time and IC/MS spectrum of the relevant peaks with those of isolated standards. The contents of salidroside and tyrosol in the samples gathered from various area in China were ranged over 1.3-11.1 mg/g and 0.3-2.2 mg/g, respectively.
建立了一种反相高效液相色谱法，用于同时测定红景天中红景天苷和酪醇的含量。最佳条件为：以Nova-Pak C18为固定相，6.5%甲醇水溶液为流动相，在紫外225 nm处检测。通过将相关峰的保留时间和离子阱质谱图与分离标准品的进行比较来进行鉴定。从中国不同地区采集的样品中，红景天苷和酪醇的含量分别在1.3-11.1 mg/g和0.3-2.2 mg/g之间。
