Germanà M A, Chiancone B
Dipartimento di Colture Arboree, Facoltà di Agraria, Viale delle Scienze 11, 90128 Palermo, Italy.
Plant Cell Rep. 2003 Oct;22(3):181-7. doi: 10.1007/s00299-003-0669-7. Epub 2003 Jul 15.
An improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4 degrees C, 25 degrees C, 32 degrees C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4 degrees C and 25 degrees C were more favorable for embryo production than 32 degrees C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time.
通过对影响柑橘花药培养单倍体诱导的多个因素进行评估，改进了克莱门氏小柑橘（Citrus clementina Hort. ex Tan. cv. Nules）花药培养的方案。研究了噻苯隆（TDZ）和三种温度预处理（4℃、25℃、32℃）对克莱门氏小柑橘（Citrus clementina Hort. ex Tan., cv. Nules）花芽花药培养的影响。添加TDZ的培养基诱导胚状体产量增加。4℃和25℃的预处理温度比32℃更有利于胚胎产生。本文首次报道了从克莱门氏小柑橘cv. SRA 63的花药培养中再生出单倍体植株。
