Froelicher Yann, Bassene Jean-Baptiste, Jedidi-Neji Emna, Dambier Dominique, Morillon Raphael, Bernardini Geneviève, Costantino Gilles, Ollitrault Patrick
Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), San Giuliano 20230, France.
Plant Cell Rep. 2007 Jul;26(7):937-44. doi: 10.1007/s00299-007-0314-y. Epub 2007 Feb 22.
This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of 'Fortune' (Citrus clementina Hort ex Tan. x Citrus tangerina Hort ex Tan.) and 'Ellendale' (Citrus reticulata Blanco x Citrus sinensis L. Osb) is reported here for the first time.
本研究聚焦于通过用辐照过的‘北京柠檬’花粉授粉进行原位雌核发育来诱导柑橘单倍体。用四种γ射线辐照水平（150、300、600和900戈瑞）的花粉对三种基因型的柑橘进行授粉。所得到的种子个头较小。在体外拯救胚胎，并通过流式细胞术分析确定幼苗的倍性水平。获得了单倍体、二倍体、三倍体幼苗。使用微卫星标记分析和染色体计数确认了单倍体孤雌生殖起源。二倍体和三倍体植株是柑橘与柠檬杂交的结果。本文首次报道了‘福琼’（克莱门氏小柑橘×红橘）和‘埃伦代尔’（宽皮橘×甜橙）雌核发育单倍体的诱导情况。
