用编码NcGRA7或NcsHSP33的质粒DNA免疫小鼠可提供对犬新孢子虫垂直传播的部分保护。
Immunization of mice with plasmid DNA coding for NcGRA7 or NcsHSP33 confers partial protection against vertical transmission of Neospora caninum.
作者信息
Liddell Susan, Parker Carolyn, Vinyard Bryan, Jenkins Mark, Dubey J P
机构信息
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA.
出版信息
J Parasitol. 2003 Jun;89(3):496-500. doi: 10.1645/GE-2969.
The purpose of the present study was to use direct plasmid deoxyribonucleic acid (DNA) injection to identify specific antigens that confer protection against congenital transfer of Neospora caninum. Inbred BALB/c mice were vaccinated before pregnancy with a recombinant plasmid containing sequences encoding N. caninum antigen NcGRA7 or NcsHSP33. The mice were challenged with N. caninum tachyzoites at 10-12 days of gestation. Whereas 100% of pups born from dams immunized with control plasmid contained detectable levels of N. caninum DNA in a Neospora-specific polymerase chain reaction assay, only 46% of pups from pCMVi-NcGRA7-immunized mice and 53% of pCMVi-NcsHSP33-immunized mice were N. caninum positive, and none of the mice immunized with tachyzoite extract contained N. caninum DNA. Thus, immunization of mice with plasmid DNA expressing N. caninum antigens conferred partial protection against congenital neosporosis.
本研究的目的是通过直接注射质粒脱氧核糖核酸（DNA）来鉴定能够提供针对犬新孢子虫先天性传播保护作用的特异性抗原。在怀孕前，用含有编码犬新孢子虫抗原NcGRA7或NcsHSP33序列的重组质粒对近交系BALB/c小鼠进行免疫接种。在妊娠10 - 12天时，用犬新孢子虫速殖子对小鼠进行攻击。在新孢子虫特异性聚合酶链反应检测中，用对照质粒免疫的母鼠所生的幼崽中有100%含有可检测水平的犬新孢子虫DNA，而用pCMVi - NcGRA7免疫的小鼠所生幼崽中只有46%为犬新孢子虫阳性，用pCMVi - NcsHSP33免疫的小鼠所生幼崽中53%为阳性，用速殖子提取物免疫的小鼠中没有一只含有犬新孢子虫DNA。因此，用表达犬新孢子虫抗原的质粒DNA免疫小鼠可对先天性新孢子虫病提供部分保护。
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