Nishikawa Yoshifumi, Zhang Houshuang, Ikehara Yuzuru, Kojima Naoya, Xuan Xuenan, Yokoyama Naoaki
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Clin Vaccine Immunol. 2009 Jun;16(6):792-7. doi: 10.1128/CVI.00032-09. Epub 2009 Apr 8.
The present study demonstrates that the subcutaneous administration of Neospora caninum dense granule protein 7 (NcGRA7) entrapped in liposomes coated with mannotriose strongly induces the parasite-specific T-helper type 1 immune response and humoral antibody in mice. Although anti-NcGRA7 immunoglobulin G1 antibody production was induced in mice injected with NcGRA7 alone, the dams and offspring were never protected from N. caninum infection. The immunization of mice with liposome-entrapped NcGRA7 before pregnancy resulted in increased offspring survival and decreased the infection rates in the brains of dams after parasite infection at 6 to 9 days of gestation. In conclusion, oligomannose-coated liposome-entrapped NcGRA7 can be used as a new type of effective vaccine to control neosporosis.
本研究表明，皮下注射包裹在甘露三糖包被脂质体中的犬新孢子虫致密颗粒蛋白7（NcGRA7）能强烈诱导小鼠产生寄生虫特异性1型辅助性T细胞免疫反应和体液抗体。虽然单独注射NcGRA7的小鼠可诱导产生抗NcGRA7免疫球蛋白G1抗体，但母鼠及其后代均未免受犬新孢子虫感染。在怀孕前用脂质体包裹的NcGRA7免疫小鼠，可提高后代存活率，并降低妊娠6至9天时感染寄生虫后母鼠大脑中的感染率。总之，甘露寡糖包被的脂质体包裹NcGRA7可作为一种新型有效疫苗用于控制新孢子虫病。
