Nakayama Satomi, Katoh Eiko M, Tsuzuki Toshi, Kobayashi Shizuko
Kyoritsu College of Pharmacy, Tokyo 105-8512, Japan.
J Invest Dermatol. 2003 Aug;121(2):406-11. doi: 10.1046/j.1523-1747.2003.12351.x.
The ability of the novel water-soluble provitamin E, alpha-tocopherol-6-O-phosphate, to protect against ultraviolet B-induced damage in cultured mouse skin was investigated and compared with the protectiveness of alpha-tocopherol acetate in cultured mouse skin. Pretreatment of skin with 0.5% (9.4 mM) alpha-tocopherol-6-O-phosphate in medium for 3 h significantly prevented such photodamage as sunburn cell formation, DNA degradation, and lipid peroxidation, which were induced in control cultured skin by a single dose of ultraviolet B irradiation at 0 to 40 kJ per m2 (290-380 nm, maximum 312 nm). This protection was greater than that seen with alpha-tocopherol acetate, the most common provitamin E that is used in commercial human skin care products. The concentration of alpha-tocopherol in cultured skin pretreated with 0.5% alpha-tocopherol-6-O-phosphate rose to approximately two to three times that found in the control skin and the reduction in cutaneous alpha-tocopherol that was induced by ultraviolet irradiation was significantly inhibited. In the group pretreated with 0.5% alpha-tocopherol acetate, however, conversion of alpha-tocopherol acetate to alpha-tocopherol was not observed, although the level of provitamin incorporated into the cultured skin was the same as that for alpha-tocopherol-6-O-phosphate. These findings indicated that the enhanced ability of alpha-tocopherol-6-O-phosphate to protect against ultraviolet B-induced skin damage compared with alpha-tocopherol acetate may have been due to alpha-tocopherol-6-O-phosphate's conversion to alpha-tocopherol. Moreover, following pretreatment with a 0.5% alpha-tocopherol-6-O-phosphate, alpha-tocopherol-6-O-phosphate was incorporated into the human skin in a three-dimensional model and 5% of the incorporated alpha-tocopherol-6-O-phosphate was converted to alpha-tocopherol. These results suggest that treatment with the novel provitamin E, alpha-tocopherol-6-O-phosphate may be useful in preventing ultraviolet-induced human skin damage.
研究了新型水溶性维生素E前体α-生育酚-6-O-磷酸酯对培养的小鼠皮肤紫外线B诱导损伤的防护能力，并与α-生育酚醋酸酯对培养的小鼠皮肤的防护能力进行了比较。用含0.5%（9.4 mM）α-生育酚-6-O-磷酸酯的培养基预处理皮肤3小时，可显著预防晒伤细胞形成、DNA降解和脂质过氧化等光损伤，这些损伤是在对照培养皮肤中由0至40 kJ每平方米（290 - 380 nm，最大312 nm）的单次紫外线B照射诱导产生的。这种保护作用比商业人体护肤品中最常用的维生素E前体α-生育酚醋酸酯的保护作用更强。用0.5%α-生育酚-6-O-磷酸酯预处理的培养皮肤中α-生育酚的浓度升至对照皮肤中浓度的约两到三倍，并且紫外线照射诱导的皮肤中α-生育酚的减少被显著抑制。然而，在0.5%α-生育酚醋酸酯预处理组中，未观察到α-生育酚醋酸酯向α-生育酚的转化，尽管掺入培养皮肤中的维生素E前体水平与α-生育酚-6-O-磷酸酯的相同。这些发现表明，与α-生育酚醋酸酯相比，α-生育酚-6-O-磷酸酯对紫外线B诱导的皮肤损伤的增强防护能力可能是由于α-生育酚-6-O-磷酸酯转化为α-生育酚。此外，用0.5%α-生育酚-6-O-磷酸酯预处理后，α-生育酚-6-O-磷酸酯被掺入三维模型的人体皮肤中，并且掺入的α-生育酚-6-O-磷酸酯中有5%转化为α-生育酚。这些结果表明，用新型维生素E前体α-生育酚-6-O-磷酸酯进行治疗可能有助于预防紫外线诱导的人体皮肤损伤。
