Lin Hong, Niu Ying-jun, Zhou Zhan-yu, Liu Fu-ling, Wang Hong-yun
Department of Ophthalmology, Affiliated Hospital, Medical College of Qingdao University, Qingdao 266003, China.
Zhonghua Yan Ke Za Zhi. 2003 Mar;39(3):167-71.
To study the distribution and expression of type IV collagen (Col IV), laminin (LN) and fibronectin (FN) in the basement membrane of malignant eyelid tumors so as to explore the relationships between the basement membrane and the degree of differentiation, metastasis and prognosis of malignant tumors.
Eighty-five paraffin-embedded specimens of malignant eyelid tumors including basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and sebaceous cell carcinoma were studied to identify the Col IV, LN and FN with immunohistochemical SABC techniques.
A continuous intact basement membrane (BM) could be identified in all of the normal tissues and benign tumor specimens. In the malignant tumor specimens, the BM was defective or absent. The positive expression rate of Col IV in BCC, SCC and sebaceous cell carcinoma was 65% (24/40), 56% (16/25) and 40% (8/20). The positive expression rate of LN was 58% (23/40), 40% (10/25) and 35.% (7/20), respectively. The positive expression rate of FN was 33% (13/40), 36% (9/25) and 30% (6/20), respectively. The positive expression rate of these extracellular matrices was correlated with the degree of differentiation of the tumor. The difference of positive rate between highly-differentiated and poorly-differentiated tumors was significant (P < 0.05). In a short term follow-up (6-18 months), a statistically significant correlation (P < 0.05) could be observed between tumor recurrence and BM integrity. A higher recurrence rate and early recurrence were found in patients with interrupted BM.
The absence of expression of Col IV, LN and FN was related to the invasive capacity and recurrence of the tumor. This result can be used as an index for clinical judgment of prognosis of the eyelid tumors.
研究IV型胶原（Col IV）、层粘连蛋白（LN）和纤连蛋白（FN）在恶性眼睑肿瘤基底膜中的分布及表达情况，以探讨基底膜与恶性肿瘤分化程度、转移及预后的关系。
采用免疫组化SABC技术，对85例石蜡包埋的恶性眼睑肿瘤标本（包括基底细胞癌、鳞状细胞癌和皮脂腺癌）进行研究，以鉴定Col IV、LN和FN。
在所有正常组织和良性肿瘤标本中均可识别出连续完整的基底膜（BM）。在恶性肿瘤标本中，BM有缺陷或缺失。基底细胞癌、鳞状细胞癌和皮脂腺癌中Col IV的阳性表达率分别为65%（24/40）、56%（16/25）和40%（8/20）。LN的阳性表达率分别为58%（23/40）、40%（10/25）和35%（7/20）。FN的阳性表达率分别为33%（13/40）、36%（9/25）和30%（6/20）。这些细胞外基质的阳性表达率与肿瘤的分化程度相关。高分化和低分化肿瘤之间的阳性率差异有统计学意义（P<0.05）。在短期随访（6-18个月）中，可观察到肿瘤复发与BM完整性之间存在统计学显著相关性（P<0.05）。BM中断的患者复发率较高且复发较早。
Col IV、LN和FN表达缺失与肿瘤的侵袭能力和复发有关。该结果可作为眼睑肿瘤临床预后判断的指标。
