Lamari Fotini N, Kuhn Reinhard, Karamanos Nikos K
Department of Chemistry, University of Patras, 261 10, Patras, Greece.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 5;793(1):15-36. doi: 10.1016/s1570-0232(03)00362-3.
Carbohydrates, either alone or as constituents of glycoproteins, proteoglycans and glycolipids, are mediators of several cellular events and (patho)physiological processes. Progress in the "glycome" project is closely related to the analytical tools used to define carbohydrate structure and correlate structure with function. Chromatography, electrophoresis and mass spectrometry are the indispensable analytical tools of the on-going research. Carbohydrate derivatization is required for most of these analytical procedures. This review article gives an overview of derivatization methods of carbohydrates for their liquid chromatographic and electrophoretic separation, as well as the mass spectrometric characterization. Pre-column and on-capillary derivatization methods are presented with special emphasis on the derivatization of large carbohydrates.
碳水化合物，无论是单独存在还是作为糖蛋白、蛋白聚糖和糖脂的组成成分，都是多种细胞事件和（病理）生理过程的介质。“糖组”项目的进展与用于确定碳水化合物结构并将结构与功能相关联的分析工具密切相关。色谱法、电泳法和质谱法是当前研究中不可或缺的分析工具。这些分析程序大多需要对碳水化合物进行衍生化。本文综述了用于碳水化合物液相色谱和电泳分离以及质谱表征的衍生化方法。介绍了柱前衍生化和毛细管上衍生化方法，特别强调了大分子碳水化合物的衍生化。
