马铃薯块茎（Solanum tuberosum L.）衰老和芽发育过程中的脂质和氧化脂质谱。
Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.).
作者信息
Fauconnier Marie Laure, Welti Ruth, Blée Elizabeth, Marlier Michel
机构信息
Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030, Gembloux, Belgium.
出版信息
Biochim Biophys Acta. 2003 Jul 21;1633(2):118-26. doi: 10.1016/s1388-1981(03)00105-7.
Potato tubers (Solanum tuberosum L. cv Bintje) were stored at 20 degrees C for 210 days without desprouting to study the lipoxygenase pathway during aging. After 15 days of storage, potato tubers sprouted, while after 45-60 days, apical dominance was lost and multiple sprouts developed. Analysis of the fatty acid hydroperoxides (HPOs) revealed that 9-S-hydroperoxide of linoleic acid (9-HPOD) was the main oxylipin formed. Between 45 and 60 days of storage, increases in the levels of 9-HPOD and colneleic acid were observed. Analysis of phospholipids and galactolipids by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) showed that a decrease in the levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), digalactosyldiacylglycerol (DGDG), and monogalactosyldiacylglycerol (MGDG) occurred between 0 and 45 days of aging. The decrease in the amount of linoleic acid in complex lipids correlates well with the amount of 9-HPOD and colneleic acid produced.
马铃薯块茎（Solanum tuberosum L. cv Bintje）在20摄氏度下储存210天，不进行去芽处理，以研究衰老过程中的脂氧合酶途径。储存15天后，马铃薯块茎发芽，而在45 - 60天后，顶端优势丧失，出现多个芽。对脂肪酸氢过氧化物（HPOs）的分析表明，亚油酸的9 - S - 氢过氧化物（9 - HPOD）是形成的主要氧脂。在储存45至60天期间，观察到9 - HPOD和可乐油酸水平升高。通过电喷雾电离串联质谱（ESI - MS/MS）对磷脂和半乳糖脂的分析表明，在衰老的0至45天之间，磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）、二半乳糖基二酰基甘油（DGDG）和单半乳糖基二酰基甘油（MGDG）的水平下降。复合脂质中亚油酸含量的下降与产生的9 - HPOD和可乐油酸的量密切相关。
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