Fauconnier Marie Laure, Welti Ruth, Blée Elizabeth, Marlier Michel
Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030, Gembloux, Belgium.
Biochim Biophys Acta. 2003 Jul 21;1633(2):118-26. doi: 10.1016/s1388-1981(03)00105-7.
Potato tubers (Solanum tuberosum L. cv Bintje) were stored at 20 degrees C for 210 days without desprouting to study the lipoxygenase pathway during aging. After 15 days of storage, potato tubers sprouted, while after 45-60 days, apical dominance was lost and multiple sprouts developed. Analysis of the fatty acid hydroperoxides (HPOs) revealed that 9-S-hydroperoxide of linoleic acid (9-HPOD) was the main oxylipin formed. Between 45 and 60 days of storage, increases in the levels of 9-HPOD and colneleic acid were observed. Analysis of phospholipids and galactolipids by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) showed that a decrease in the levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), digalactosyldiacylglycerol (DGDG), and monogalactosyldiacylglycerol (MGDG) occurred between 0 and 45 days of aging. The decrease in the amount of linoleic acid in complex lipids correlates well with the amount of 9-HPOD and colneleic acid produced.
马铃薯块茎（Solanum tuberosum L. cv Bintje）在20摄氏度下储存210天，不进行去芽处理，以研究衰老过程中的脂氧合酶途径。储存15天后，马铃薯块茎发芽，而在45 - 60天后，顶端优势丧失，出现多个芽。对脂肪酸氢过氧化物（HPOs）的分析表明，亚油酸的9 - S - 氢过氧化物（9 - HPOD）是形成的主要氧脂。在储存45至60天期间，观察到9 - HPOD和可乐油酸水平升高。通过电喷雾电离串联质谱（ESI - MS/MS）对磷脂和半乳糖脂的分析表明，在衰老的0至45天之间，磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）、二半乳糖基二酰基甘油（DGDG）和单半乳糖基二酰基甘油（MGDG）的水平下降。复合脂质中亚油酸含量的下降与产生的9 - HPOD和可乐油酸的量密切相关。
