Fauconnier Marie-Laure, Williams Todd D, Marlier Michel, Welti Ruth
Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030 Gembloux, Belgium.
FEBS Lett. 2003 Mar 13;538(1-3):155-8. doi: 10.1016/s0014-5793(03)00171-6.
Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemical hydrolysis and a phospholipase A(2), but not by a lipase with 1-acyl specificity. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol contain molecular species with nominal masses consistent with identification as palmitoyl,colneleoyl species. Exact mass analysis of its fragments confirmed the identity of palmitoyl,colneloyl phosphatidylinositol. To our knowledge, this work represents the first identification of a colneleoyl phospholipid.
结肠油酸（9-[1'(E),3'(Z)-壬二烯氧基]-8(E)-壬烯酸）由亚油酸通过9-脂氧合酶和二乙烯基醚合酶的顺序作用产生。我们证明，马铃薯块茎中一小部分结肠油酸以磷脂形式酯化。这种结肠油酸可通过化学水解和磷脂酶A(2)释放，但具有1-酰基特异性的脂肪酶不能释放。磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇含有标称质量与鉴定为棕榈酰、结肠油酰种类一致的分子种类。对其片段的精确质量分析证实了棕榈酰、结肠油酰磷脂酰肌醇的身份。据我们所知，这项工作代表了首次鉴定出结肠油酰磷脂。
