通过快速实时逆转录聚合酶链反应检测持续感染口蹄疫病毒的携带病毒牛羊。
Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay.
作者信息
Zhang Zhidong, Alexandersen Soren
机构信息
Pirbright Laboratory, Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK.
出版信息
J Virol Methods. 2003 Aug;111(2):95-100. doi: 10.1016/s0166-0934(03)00165-4.
The detection of foot-and-mouth disease virus (FMDV) in persistently infected carriers among exposed ruminants is of great importance in disease control. For this purpose, a real time, fluorogenic reverse transcription polymerase chain reaction (real-time RT-PCR) assay was evaluated for the identification of FMDV carrier animals. The results indicate that this real time RT-PCR assay may be suitable for detection of FMDV carrier animals.
在暴露的反刍动物中检测持续感染口蹄疫病毒（FMDV）的携带者对于疾病控制至关重要。为此，对一种实时荧光逆转录聚合酶链反应（实时RT-PCR）检测方法进行了评估，以鉴定口蹄疫病毒携带者动物。结果表明，这种实时RT-PCR检测方法可能适用于检测口蹄疫病毒携带者动物。
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