Zhang Zhidong, Alexandersen Soren
Pirbright Laboratory, Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK.
J Virol Methods. 2003 Aug;111(2):95-100. doi: 10.1016/s0166-0934(03)00165-4.
The detection of foot-and-mouth disease virus (FMDV) in persistently infected carriers among exposed ruminants is of great importance in disease control. For this purpose, a real time, fluorogenic reverse transcription polymerase chain reaction (real-time RT-PCR) assay was evaluated for the identification of FMDV carrier animals. The results indicate that this real time RT-PCR assay may be suitable for detection of FMDV carrier animals.
在暴露的反刍动物中检测持续感染口蹄疫病毒（FMDV）的携带者对于疾病控制至关重要。为此，对一种实时荧光逆转录聚合酶链反应（实时RT-PCR）检测方法进行了评估，以鉴定口蹄疫病毒携带者动物。结果表明，这种实时RT-PCR检测方法可能适用于检测口蹄疫病毒携带者动物。
